Science Hub

Welcome to Nordic BioSite's Life Science Blog. Here, we will provide commentary on current events and trends in biological sciences, interesting stories about important scientists, technical tips and suggestions, and much more. Read on and learn something new.
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The Typical Immunohistochemistry Workflow

Immunohistochemistry (IHC) ties together immunological, biochemical and microscopic techniques to yield visual information about proteins and other macromolecules in tissues. Targets are detected through the use of labelled antibodies that penetrate tissues and specifically bind proteins and other cellular components of interest, e.g., organelles.

RNA-Seq 3: Technology Overview

Welcome back to our RNA-seq article series! Now that we’ve gone through the general workflow and the ins and outs of RNA quality, we will look at some of the popular methods and platforms that you are likely to encounter if you embark on RNA-seq experiments.

Parkinson’s Disease & The Microbiome

Parkinson’s disease (PD) is a debilitating and incurable age-related neurodegenerative disease that affects bodily movements and worsens over time. It is one of the most common neurological diseases and its incidence is rising rapidly, with 14 million cases expected globally just 20 years from now.

World AIDs Day 2019

Yesterday was an important day for Christians all over the world. The first Advent candles were lit and millions of children started preparing their annual letters to Santa.

But did you know that yesterday was also World AIDS Day? In fact, it was the 31st AIDS day since the tradition started in 1988, when AIDSs was the first disease to get its own Global Health Day.

RNA-Seq 2: RNA Quality

Welcome back to our RNA-seq series! In Part 2, we take a look at RNA quality considerations for RNA-seq.

As outlined in Part 1, library preparation is a pivotal part of the RNA-seq workflow. Although the exact protocol will vary according to RNA-seq platform technology (more about this in Part 3), the overall goal is the same: to create a library of complementary DNA fragments that represent the originating RNA sample as accurately as possible and provide information about the RNA features of interest, including information about antisense transcripts, alternative splice variants, miRNAs and other non-coding RNAs, low-abundance transcripts and more.

Antibody Validation 101

Many researchers start their hunt for new antibodies by scouring the literature to find out which antibodies others are using for the same target. While this is a reasonable starting point, simply choosing an antibody with reported target specificity and sensitivity is not enough, even if the published data looks very convincing! In practice, the suitability of an antibody for a given sample type, experimental conditions, and application depends on many more parameters than affinity for a target protein.