NEW! Oxytocin CLIA kit measures Oxytocin and Mesotocin. Worlds most sensitive kit for Oxytocin.
The neuropeptides, oxytocin and vasopressin were isolated and synthesized by Vincent du Vigneaud at Cornel Medical College in 1953, work for which he received the Nobel Prize in Chemistry in 1955. Oxytocin is a neurohypophysial peptide which is produced in the paraventricular nuclei of the hypothalamus and stored in the posterior pituitary. The molecule consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. A hormone once thought to be limited to female smooth muscle reproductive physiology and neurotransmitter (1,2), recent studies have begun to investigate oxytocin’s role in various behaviors,including orgasm, social recognition, pair bonding, anxiety, and maternal behaviors (3,4) and is important in male reproductive physiology (5). Oxytocin and the related neurohypophysial peptide, Arg8-Vasopressin, maintain renal water and sodium balance (6).
Highly conserved across species boundaries, oxytocin-like neurohypophysial peptides are substituted primarily at residues 4 and/or 8. In the oxytocin-like peptide, mesotocin, a common peptide found in some fishes, reptiles, birds, amphibians, marsupials and non-mammalian tetrapods, the leucine at residue 8 is substituted for isoleucine(7). Acting in classical endocrine fashion, Oxytocin elicits regulatory effects by binding specific cell surface receptors which in turn initiate a secondary intracelluar response cascade via a phosphoinositide signaling pathway.
Our New Oxytocin Chemiluminescent Immunoassay, Catalog Numbers K048-C1 (One Plate) and K048-C5 (Five Plate), breaks the 10 pg/mL barrier. It is designed to quantitatively measure Oxytocin present in serum, plasma, clarified milk and tissue culture media samples. An oxytocin standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a white microtiter plate coated with an antibody to capture rabbit antibodies. An oxytocin-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to oxytocin to each well. After an overnight incubation at 4°C the plate is washed and substrate is added. The substrate reacts with the bound oxytocin-peroxidase conjugate to produce light. The intensity of the generated chemiluminescent signal is detected in a microtiter plate reader capable of measuring luminescence.
MULTI-SPECIES – 88.4% Reactivity to Mesotocin
SENSITIVITY – >2 Times the Sensitivity of Any Other Kit
SIMPLE – No C18 Column Purifications
INEXPENSIVE – Extraction Solution Included
SAMPLES/KIT – 38 or 230 in Duplicate
SAMPLES – Mammalian, Bird, Fish
STABLE – 4°C Stability