Formulated for Loop-Mediated Isothermal DNA Amplification (LAMP)
Meridian has just released two new Bst DNA polymerases that work with an optimized buffer system to enhance an assay’s amplification speed, yield, salt tolerance and sensitivity. The enzymes are derived from the large fragment of Bacillus stearothermophilus DNA Polymerase I and contain 5´- 3´ DNA polymerase activity and strong strand displacement activity, but lack 5´- 3´ exonuclease activity. The strong strand displacement activity enables the enzymes to synthesize DNA at a constant temperature making them ideal for Loop- Mediated Isothermal DNA Amplification (LAMP).
Product Highlights:
- Flexible reaction conditions including high salinity
- Fast polymerization
- Optimized for Loop-Mediated Isothermal DNA Amplification (LAMP)
- Reaction buffer and dilution buffers sold separately for maximum flexibility
High Salt Tolerance
LAMP reactions were performed with Meridian Bst DNA Polymerase and Bst from supplier B using increasing amounts of salt (potassium chloride) and the manufacturers recommended protocol. The time to results (TTR) illustrate that Meridian Bst DNA Polymerase is as resistant to inhibitors as supplier B’s Bst.