Preserving blood for liquid biopsy research is particularly difficult due to high fragility of blood cells, leaking cellular DNA and RNA into cell-free plasma. Additionally, denaturing extracellular environments in blood can lead to degradation of cell-free DNA and cell-free RNA.
The cfDNA/cfRNA Preservation Blood TubeTM provides preservation of both cfDNA and cfRNA at ambient temperatures (15-25 °C) for up to 7 days without harsh crosslinking preservative chemicals that may negatively impact downstream assays.
Up to 55x less gDNA contamination compared to unpreserved blood
Assessment of increasing gDNA contamination in blood tubes over time. Contamination from white blood cell lysis observed after 2 days in unprotected blood (EDTA) versus contamination-free in preserved blood (ZYMO), measured by Qubit dsDNa High Sensitivity.
Achieve up to 18x better cfRNA preservation compared to other cfRNA preservation technology
Assessment of increasing cellular RNA contamination in blood tubes over time, compared to immediate processing (2 hours). Increasing amounts of contaminant miRNA (miR-16) of white blood cell origin observed in unpreserved (EDTA) and Streck RNA BCT (ST-RNA) versus Zymo preserved blood, measured by RT-qPCR.
Read more about Zymo here.