Illuminating the Way for RNA Methylation Studies

news June 30 2021
RNA Methylation Studies

 

First-Rate RNA Methylation Research Kits

When it comes to the study of RNA methylation, EpiGentek is a beacon of light that can help guide your research in the right direction. From quickly isolating even the smallest amounts of RNA to enriching and capturing m6A containing RNA fragments for next-generation sequencing, we make it easy and affordable. Their RNA methylation kits are widely used and have been cited in numerous studies, plus they are backed by EpiGentek’s 100% satisfaction guarantee, assuring smooth sailing on your project.

See Which RNA Methylation Kit Goes With Your Study

Rapid, Pure, and Simple RNA Isolation Measure m6A or 5mC RNA Amounts Get a Clear View of m6A Activity Levels Enrich and Capture m6A RNA

 

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RNA Extraction Kits

Rapid, Pure, and Simple RNA Isolation

A few notable benefits include:

  • Fast procedure delivers high-quality total RNA approximately 30 minutes
  • Ready-to-use RNA for high performance in downstream applications
  • Starting materials can be up to 1 x 106 mammalian cells or 10 mg tissues
Art#
Name
Description
EpiQuik Total RNA Isolation Fast Kit
Isolate total RNA in cells or tissues using a column-based format in about 30 minutes
EpiQuik Magbeads Quick RNA Isolation Kit
Isolate total RNA in cells or tissues using a magnetic beads format in about 30 minutes

 


Global m6A & 5mC RNA Quantification Kits

Measure m6A or 5mC RNA Amounts

A few notable benefits include:

  • Fast, easy-to-follow ELISA-like kits
  • Detection as low as 5 pg of m6A or 0.02% of 5mC RNA from 200 ng of input RNA
  • High specificity to m6A or 5-mC with no cross-reactivity to unmethylated adenosine or cytosine or hydroxymethylated cytosine
  • Manual or high throughput capabilities
Art#
Name
Description
EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Colorimetrically quantify N6-methyladenosine (m6A/6mA) in RNA in less than 4 hours
EpiQuik m6A RNA Methylation Quantification Kit (Fluorometric)
Fluorometrically quantify N6-methyladenosine (m6A/6mA) in RNA in less than 4 hours
MethylFlash Urine m6A Quantification Kit
Quantify N6-methyladenosine in urine via an ELISA-like format in 4 hours
MethylFlash 5-mC RNA Methylation ELISA Easy Kit
Quantify 5-mC in RNA in 2 hours and 40 minutes

 


m6A Methylase & Demethylase Activity Assays

Get a Clear View of m6A Activity Levels

A few notable benefits include:

  • Fast, easy-to-follow ELISA-like kits
  • Detection as low as 2 µg of nuclear extracts or 50 ng of purified enzymes
  • Includes assay standard for specific measurement of m6A demethylase or methylase activity
  • Manual or high throughput capabilities
Art#
Name
Description
Epigenase m6A Demethylase Activity/Inhibition Assay Kit
Measure total activity/inhibition of m6A demethylase from nuclear extracts or purified enzymes within 5 hours
Epigenase m6A Methylase Activity/Inhibition Assay Kit
Measure total activity/inhibition of m6A methylases from nuclear extracts or purified enzymes within 5 hours

 


MeRIP & m6A-seq Assays

Enrich and Capture m6A RNA

A few notable benefits include:

  • Generates short RNA fragments bound only to m6A antibody
  • Input RNA can be as low as 500 ng
  • Data analysis with <10 million reads
Art#
Name
Description
EpiQuik CUT&RUN m6A RNA Enrichment Kit
MeRIP based on CUT&RUN for increased specificity and signals
EpiNext CUT&RUN RNA m6A-Seq Kit
All-in-one MeRIP and NGS library preparation based on CUT&RUN

 

To offer you a complete repertoire of tools to use during your RNA methylation studies, EpiGentek also provides the following for your convenience: