Latest News from Meridian Life Science

news October 19 2021

 

New Air-Dryable™ Direct Blood Mixes

For creating ambient-temperature stable qPCR/RT-qPCR assays capable of direct amplification from blood samples

 

Air-Dryable™ Direct DNA qPCR Blood and Air-Dryable™ Direct RNA/DNA qPCR Blood mixes are glycerol-free and contain optimized excipients that are compatible with air and oven drying. The mixes have been designed for the direct detection of DNA and RNA from whole blood, serum or plasma without extraction.

Product Highlights:

  • Inhibitor-tolerant and designed for the direct detection of viruses, bacteria and cell-free nucleic acids at very low titers from crude blood samples
  • Suitable for singleplex or multiplex assays
  • Mixes can be used as a liquid or dry format, reducing the cost and complexity of creating ambient-temperature stable assays
  • Compatible with a range of air-drying protocols
Product Cat No Volume Reactions
Air-Dryable™ Direct DNA qPCR Blood MDX092 5 mL 1,00 Rxn
50 mL 10,000 Rxn
Air-Dryable™ Direct RNA/DNA qPCR Blood MDX121 – inquire 5 mL 1,00 Rxn
50 mL 10,000 Rxn

 

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New Air-Dryable Direct Stool Mixes

For creating ambient-temperature stable qPCR/RT-qPCR assays capable of direct amplification from stool samples

 

Air-Dryable™ Direct DNA qPCR and RNA/DNA qPCR Stool mixes are glycerol-free and contain optimized excipients compatible with air and oven drying. Key features include:

  • Simple and easy to use
  • Suitable for singleplex or multiplex assays detecting RNA or DNA pathogens and cancerous material at very low titers from stool samples
  • Mixes can be used in a wet or dry format, reducing the cost and complexity of developing an ambient-temperate stable assay
  • Compatible with a range of air-drying protocols
Product Cat No Volume Reactions
Air-Dryable™ Direct DNA qPCR Stool MDX140 5 mL 1,00 Rxn
50 mL 10,000 Rxn
Air-Dryable™ Direct RNA/DNA qPCR Stool MDX141 5 mL 1,00 Rxn
50 mL 10,000 Rxn

 

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Sensitive detection in multiplex assays in the presence of stool inhibitors

Adenovirus and three other targets not shown (Rotavirus, Norovirus and Campylobacter jejuni) were amplified in a quadruplex reaction using Air-Dryable™ Direct RNA/DNA qPCR Stool (blue) or mixes from supplier T (red) or supplier Q (grey) in the presence of 1.5 mg/mL bile salts. The results illustrate that Air-Dryable Direct RNA/DNA qPCR Stool is less affected by increased concentrations of bile, unlike supplier T and Q mixes.

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