LiveReceptor AMPAR
LiveReceptor AMPAR is the reagent which can specifically label AMPA-type glutamate receptors (AMPARs) with fluorescein dye on living neurons.
This reagent is based on a unique chemical reaction and can accomplish labeling of AMPARs keeping its ligand-binding pockets and receptor functions.
LiveReceptor AMPAR is a powerful tool to investigate physiological functions of AMPAR by monitoring receptor trafficking of endogenous AMPARs on live cells. No complicated protocol and genetic manipulation are required.
Generally, fluorescein direct conjugated ligand has been used for labeling receptors, however, such ligands easiliy detach from ligand binding pocket, and then signal will be faded out. (See below image).
“LiveReceptor” has a linker of acyl imidazole between fluorescein and ligand. While ligand is binding to receptor, nucleophillic amino acids attacks acyl imidazole and changes its chemical structure. By this method, receptors are directly labeled and keep fluorescence even after the ligand is detached from binding pocket.
This product is commercialized by the research result of Professor Hamachi, at Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University.
- Can label fluorecein to endogenous AMPAR
- Highly specific to AMPAR (GluA2-4 subunit). Other ion channel type glutamate receptors will not be labeled.
- Can observe signal for 1 to 4 hours after adding LiveReceptor to medium.
- No cell permeability – only AMPAR on cell surface can be labeled.
- Ion channel function is maintained after labeling
- Low cytotoxicity under recommended concentration (1 μM)
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LipiDye II
LipiDye II is a high sensitive, low cytotoxic and super-photostable fluorescent dye for Lipid Droplets (LDs). LipiDye II enables to detect very small LDs (less than 1µm) and to perform long-term time-lapse imaging, including Z-stack imaging. This novel dye succeeded in observation of dynamic biosynthesis, degradation and movement of LDs in live cells.
Features
- High S/N ratio: Low background in cytoplasm via following two features
1) Specific accumulation into LDs
2) Strong green fluorecent emission in non-polar oil phase like LDs - High sensitivity: Can observe even very small (<1 μm) LDs
- Super photo-stability: Long-term live time lapse imaging is possible
- Low cytotoxicity (at the recommended concentration (0.1～1μM))
- Compatible with both live and fixed cell
- Applicable to STED microscopy: can detect less than 500 nm LDs in HeLa cells.
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ULTRARIPA kit for Lipid Raft
PA buffer is one of the most useful buffers for protein extraction. RIPA buffer maintains most native structures of proteins and the extracted proteins can be applied to various applications. However, RIPA buffer is not sufficient to extract membrane proteins and membrane-associated proteins concentrated in lipid raft.
Lipid raft is a highly specialized microdomain on the lipid bilayer which contains specialized lipids, cholesterol and functional proteins. These lipid rafts are also called “Detergent Resistant Membrane (DRM)”, as lipid raft-enriched proteins are usually insoluble by mild detergent buffers such as 1% Triton X-100 and RIPA buffer. Consequently, it was difficult to analyze functions of lipid raft-enriched proteins extracted with RIPA buffer.
BioDynamics Laboratory’s newly developed product:the ULTRARIPA kit, can efficiently and rapidly extract membrane proteins or membrane-associated proteins enriched in lipid rafts, with little effect on protein structures and functions.
ULTRARIPA kit includes a totally new buffer not containing protein denaturing detergents, but can extract the DRM which was difficult to extract by conventional RIPA buffer. ULTRARIPA kit helps to analyze various biological assays of the proteins in lipid raft.
ThelioKeep
For preserving dermal and nerve tissues in the best condition.
Features
- Maintain the morphology and proliferative ability of epithelium, endothelium and nerve tissues.
- Tissues in ThelioKeep can be preserved for 1 – 2 weeks at 4C
- Tissues kept in ThelioKeep can be used for various applications.
- Serum-free, DMSO-free and Protein-free formulation.
- Free of bacteria, fungi and mycoplasma contamination.
- The product is stable for 1 years at 4°C after the date of manufacture.
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SOD1 mutant specific antibody
New powerful tools to research pathophysiological role of SOD1 mutants in ALS patients.
Features
- Specific to SOD1 mutants
- Application ;
- IP and IC : Detect SOD1 mutant
- ELISA : Detect SOD1 mutant
- Western Blot : Detect SOD1 wild and mutant type.
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