LEGENDplex™ – bead-based flow assay kits

BioLegend’s LEGENDplex™ is a bead-based immunoassay that utilizes the same basic principles as sandwich immunoassays, whereby a soluble analyte is captured between two antibodies.  The concept allows you to quantify the levels of up to 13 substances simultaneously.

BioLegend’s LEGENDplex™ kits are provided as predefined panels, ranging from 3 to 13 specificities, or customers can mix and match any subset within each predefined panel using our Mix and Match system. If you would like to combine specificities across different predefined panels, i.e., Human Cytokine and Human Chemokine, please visit our custom panels tab at the top of this page.

Each kit provides sufficient reagents for at least 100 tests. The assay can be performed in a 96-well filter plate, allowing you to run 40 samples plus 8 standard curve titrations in duplicate, or they can be performed in FACS tubes, where duplicate titrations may not be necessary.

For analysis of LEGENDplex™ assays, BioLegend provides both PC and Mac versions of the LEGENDplex™ data analysis software free of charge. To download the software, click on the Software tab above. Note: full use of software functions requires a USB dongle (included in each kit) for PC users, or a no cost license key (see installation guide) for Mac users.

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