Non-Invasive Reproductive Biomarker ELISAs

news December 02 2019

In reproductive medicine, biomarkers are used to test fertility, track pregnancy, predict parturition, and measure the well-being of offspring. Reproductive status of vertebrates can be established and monitored through analysis of several important biomarkers, often non-invasively. The use of biomarkers to monitor the reproductive status of endangered and captive species is of paramount importance for the preservation of wildlife in the Anthropocene. Improved understanding can lead to new treatments for reproductive disorders, such as infertility. Arbor Assays makes an extensive range of immunoassays, detection, and activity assays to measure the status of critical reproductive biomarkers.

Estrogens and Progestins

Commonly measured fertility markers include steroids like Estradiol (E2) and Progesterone (P4). E2 and P4 induce ovulation, prepare for embryonic implantation, and are essential for maintaining pregnancy. Clinical studies have also demonstrated the utility of measuring the E2 and P4 metabolites Estrone-3-Glucuronide (E1G) and Pregnanediol-3-Glucuronide (PDG) as non-invasive urinary markers to monitor ovarian function. Our estradiol and progesterone assay kits are the industry standard for researchers studying the reproductive status of almost any species.

Allopregnanolone and PGFM

Allopregnanolone (Allo) is involved in neuronal plasticity and modulation of responses to stress, anxiety, and depression. Elevated Allo levels during pregnancy can predict impending parturition making it a useful marker to predict delivery. Likewise, 13,14,Dihydro-15-keto-Prostaglandin F2α (PGFM) levels are closely associated with pregnancy progression, making it a practical reproductive status biomarker.

Prolactin

Prolactin (PRL) has multiple roles in reproduction, lactation, and metabolism. Abnormal PRL production is implicated in fertility reduction and pregnancy maintenance failure. PRL levels increase predictably during pregnancy as PRL signaling regulates growth and differentiation of tissues essential for fetal development and parturition.

Ceruloplasmin

Ceruloplasmin (Cp) is a ferroxidase enzyme and major copper-carrying protein in the blood.  Elevated activity of Cp is observed in pregnancy, in likely response to associated increases in oxidative stress. In 2010, Willis et.al. observed a significant correlation between Cp activity and pregnancy status in Giant Pandas.

Arbor Assays ELISA Kits

  • High Sensitivity
  • Species Independent
  • Non-Invasive Sampling
  • Reproducible & Consistent Results
  • Cited Extensively in Peer-Reviewed Journal Articles