Oxidative Stress Damages DNA
Oxidative stress is an inevitable byproduct of cellular metabolism and is frequently associated with carcinogenesis, neurological disorders and cardiovascular disease.
Reactive oxygen species (ROS) produced during normal cellular metabolism often cause oxidative damage to proteins and DNA. Unchecked oxidative damage to DNA is a significant factor in the pathophysiology of age-related diseases.
Oxidative DNA damage is normally repaired by base excision repair mechanisms producing the 8-hydroxy-2’-deoxyguanosine product (8-OHdG). The formation of the 8-OHdG metabolite is commonly observed in systems under oxidative pressure, and quantitation of 8-OHdG is used as a measurement of DNA damage.
The Arbor Assays DetectX® DNA Damage ELISA Kit (K059-H1/H5) quantitatively measures oxidized guanosine species produced during repair of DNA and RNA oxidation. The assay detects all three oxidized guanine species: 8-OHdG from DNA; 8-hydroxyguanosine from RNA; and 8-hydroxyguanine from DNA or RNA damaged by ionizing radiation, or ROS interactions. These markers of oxidative stress may be present in serum, plasma, saliva, urine, dried fecal matter, cells and tissues.
Our DNA Damage ELISA Kits are the most sensitive 8-OHdG immunoassays available, delivering exceptionally reproducible results for quantitation of DNA oxidation biomarkers in your research!