Omicron (Pango lineage B.1.1.529) was newly classified as a variant of concern (VOC) by WHO on 26 November 2021 due to concerns regarding potential immune escape, increased transmissibility, reduction in vaccine effectiveness, as well as increased risk for re-infections compared to the Delta variant.
Mutations in the spike glycoprotein of Omicron
Sino Biological is developing a series of recombinant Omicron Spike Proteins to support the study of Omicron variant. The Omicron variant is by far the most divergent variant characterised by a high number of mutations in the spike protein. Several mutations (e.g.: N501Y, P681H) have been identified in previous variants and are associated with increased transmissibility, immune escape, or other properties.
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• 30 amino acid changes: among which 15 are located in the RBD region
• Three deletions
• Three insertion in the spike protein NTD
Sino Biological is developing a series of recombinant Omicron Spike Proteins to support the study of Omicron variant.
| Cat# | Molecules | Host | Tag | Mutants |
|---|---|---|---|---|
| 40592-V08H121 | RBD | HEK293 | C-His | G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H |
| 40592-V49H7 | RBD | HEK293 | C-His-AVI | G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H |
| 40592-V49H7-B | RBD | HEK293 | C-His-AVI | G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H |
| 40591-V08H41 | S1 | HEK293 | C-His | A67V, H69del, V70del, T95I, G142D, V143del, Y144del, Y145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H |
| 40589-V08H26 | S-ECD-Trimer | HEK293 | C-His | A67V, H69del, V70del, T95I, G142D, V143del, Y144del, Y145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F |
| 40589-V49H3-B | S-ECD-Trimer | HEK293 | C-His-AVI | A67V, H69del, V70del, T95I, G142D, V143del, Y144del, Y145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F |
| 40589-V08B33 | S-ECD | Insect | C-His | A67V, H69del, V70del, T95I, G142D, V143del, Y144del, Y145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F |
| 40588-V07E34 | N | E.coli | N-His | P13L, E31del, R32del, S33del, R203K, G204R |
SEC-MALS Validated SARS-CoV-2 Prefusion Trimeric Spike Variant Proteins
The spike protein resides on the surface of SARS-CoV-2 virions in a trimeric conformation (also known as the pre-fusion format). Evidence showed that pre-fusion Spike trimer is relatively unstable. It is essential to develop a stable pre-fusion spike protein trimer to support the development of vaccines, therapeutic antibodies, and diagnostic reagents. Sino Biological has successfully developed a series of highly stable trimeric spike proteins, covering multiple VOIs or VOCs, such as Alpha (B.1.1.7), Beta (B.1.351), Delta (B.1.617. 2), Kappa (B.1.617.1), etc.
| Cat# | WHO Label | Product Name | Mutations |
|---|---|---|---|
| 40589-V08H10 | Delta | SARS-CoV-2 B.1.617.2 Spike S1+S2 trimer Protein (ECD, His tag) | T19R, E156G, 157Del, 158Del, L452R, T478K, D614G, D950N, F817P, A892P, A899P, A942P, K986P, V987P and furin cleavage site mutants |
| 40589-V08H11 | Kappa | SARS-CoV-2 B.1.617.1 Spike S1+S2 trimer Protein (ECD, His tag) | G142D, E154K, L452R, E484Q, D614G, F817P, A892P, A899P, A942P, K986P, V987P, Q1070H, H1101D and furin cleavage site mutants |
| 40589-V08H12 | Alpha | SARS-CoV-2 B.1.1.7 Spike S1+S2 trimer Protein (ECD, His tag) | (69-70, 145 deletion), N501Y, A570D, D614G, T716I, F817P, A892P, A899P, A942P, S982A, K986P, V987P, D1118H and furin cleavage site mutants |
| 40589-V08H13 | Beta | SARS-CoV-2 B.1.351 Spike S1+S2 trimer Protein (ECD, His tag) | L18F, D80A, D215G, 241-243 deletion, R246I, K417N, E484K, N501Y, D614G, A701V, F817P, A892P, A899P, A942P, K986P, V987P and furin cleavage site mutants |
| 40589-V08H8 | SARS-CoV-2 Spike S1+S2 (D614G) trimer Protein (ECD, His tag) | D614G, F817P, A892P, A899P, A942P, K986P, V987P and furin cleavage site mutants | |
| 40589-V08H9 | SARS-CoV-2 B.1.618 Spike S1+S2 trimer Protein (ECD, His tag) | H49Y, 145-146 deletion, E484K, D614G, F817P, A892P, A899P, A942P, K986P, V987P and furin cleavage site mutants |
200+ Recombinant SARS-CoV-2 Variants
| Mutations in: RBD, S1, S2, S1+S2 ECD, Nucleocapsid | Various Fusion Tag: • His • mFc, hFc, rFc • AVI |
| Covering New Lineages: | ||
|---|---|---|
| • Alpha (B.1.1.7) • Delta (B.1.617.2, AY.1, AY.2, AY.3) • Iota (B.1.526) • Mu (B.1.621) |
• Beta (B.1.351, B.1.351.2, B.1.351.3) • Epsilon (B.1.427/429) • Kappa (B.1.617.1) • Omicron (B.1.1.529) |
• Gamma (P.1) • Eta (B.1.525) • Lambda (C.37) |