Reversible Fluorescent Dye for Cell Nucleus
NucleoSeeing is a novel live cell imaging probe which emits green fluorescence when binding specifically to DNA. NucleoSeeing can be used in animal cells or tissues, but also in e.g. Guard cell of Arabidopsis thaliana. Moreover, NucleoSeeing can be used as a pH sensor in nucleus.
This product has been commercialized under the license from Nagoya Institute of Technology.
NucleoSeeing is composed of green fluorescent dye and a DNA binding tag. Under DNA free conditions, the probe is in folded conformation and not fluorescent. Once it binds to DNA, the conformation will change and a green fluorescence signal will be emitted.
Comparison table of dyes for nucleus
|Ex/Em||Fluorescence||Photo-toxicity||Nucleus Specificity||Cell Mem. Permeability||Cyto-toxicity||Live||Fixed|
・ Excitation / Emission : 488 nm / 520 nm
・ High S/N ratio
・ Low cytotoxicity
・ Can be used for both live and fixed cells
・ Can be used for animal samples and plant cells.
(validated in leaf and guard cell of A. thaliana)
・ Reversible : Can be washed out by replacing the medium
・ Can be used as nucleus pH sensor
Nakamura, A., et al., Chem. Commun., 50, 6149-6152 (2014) Hoechst tagging: a modular strategy to design synthetic fluorescent probes for live-cell nucleus imaging.
Ueda, M., et al., ACS Cent. Sci., 3 (5), 462-472 (2017) Noncanonical function of a small-molecular virulence factor coronatine against plant immunity: an in vivo raman imaging
Nakamura, A. and Tsukiji, S., Bioorg. Med. Chem. Lett., 27 (14), 3127-3130 (2017) Ratiometric fluorescence imaging of nuclear pH in living cells using hoechst-tagged fluorescein.
* hoeAc2FL in above 3 papers is equal to NucleoSeeing.