Introduction to SensiFAST
Video: The SensiFAST Family – Overview, Features & Benefits
Probe
SensiFAST™ Probe has been developed for fast, highly specific and reproducible real-time PCR and has been validated on all commonly used real-time instruments. The kit has been formulated for use with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that the SensiFAST Probe Kit delivers fast, reproducible, highly-specific and ultra-sensitive real-time PCR, making SensiFAST Probe ideal for multiplexing.
- Highly specific – minimal non-specific activity leading to better efficiency
- Ultra-sensitive – perfect for low copy number samples
- Fast – unique buffer chemistry allowing fast protocols and higher productivity
- Efficient multiplexing – no loss in efficiency using multiple probes
SensiFAST Probe Hi-ROX Kit
SensiFAST Probe Lo-ROX Kit
SensiFAST Probe No-ROX Kit
SYBR
SensiFAST™ SYBR Kits are a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensuring that the SensiFAST SYBR Kits produces fast, highly-specific, reproducible and ultra-sensitive real-time PCR. For ease-of-use and added convenience, the kits are provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilisers and enhancers.
- Accurate quantification – hot-start capability reduces primer-dimer formation and saves time
- Sensitive – from low copy targets
- Rapid – unique buffer chemistry for highest specificity and sensitivity
- Flexible – compatible with all standard and fast cycling instruments
SensiFAST SYBR & Fluorescein Kit
SensiFAST SYBR Hi-ROX Kit
SensiFAST SYBR Lo-ROX Kit
SensiFAST SYBR No-ROX Kit
SYBR One-Step
SensiFAST™ SYBR One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The SensiFAST SYBR One-Step Kits uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. SensiFAST One-Step Kits consist of a 2x SensiFAST SYBR One-Step mix, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.
- Accurate quantification – hot-start capability reduces primer-dimer formation and saves time
- Sensitive – from low copy targets
- Rapid – unique buffer chemistry for highest specificity and sensitivity
- Flexible – compatible with all standard and fast cycling instruments
SensiFAST SYBR & Fluorescein One-Step Kit
SensiFAST SYBR Hi-ROX One-Step Kit
SensiFAST SYBR Lo-ROX One-Step Kit
SensiFAST SYBR No-ROX One-Step Kit
Probe One-Step
SensiFAST™ Probe One-Step Kits have been developed for fast RT-qPCR and has been validated on all commonly used real-time instruments. The kit is designed for superior sensitivity and specificity with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes. The SensiFAST Probe One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube and uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. This also gives SensiFAST Probe One-Step unbeatable efficiency in multiplexing. SensiFAST Probe One-Step Kits consists of a 2x SensiFAST Probe One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.
- Rapid – optimized for fast reverse transcription real-time PCR
- Accurate quantification – for calculating gene expression from RNA
- Sensitive – unique buffer chemistry gives superior sensitivity of low copy targets
- Flexible – compatible with all standard and fast cycling instruments
SensiFAST Probe Hi-ROX One-Step Kit
SensiFAST Probe Lo-ROX One-Step Kit
SensiFAST Probe No-ROX One-Step Kit
Genotyping
Single nucleotide polymorphisms (SNPs) are single-base variations at specific sites in the genome. The frequency of SNPs is about one in 300bp in the human genome, making it one of the most frequent sequence variations. High resolution melt (HRM) and duel probe-based analysis, are examples of methods used for SNP genotyping. SensiFAST HRM Kit is a post-PCR analysis method of characterizing nucleic acid samples based on DNA strand dissociation behaviour during transition from double-stranded DNA to single-stranded DNA with increasing temperature and is low throughput. SensiFAST Genotyping Kit uses a dual-labeled probe assay fast, precise and highly reproducible genotyping and can be used for high throughput.
- Accurate allele calling – clear allele discrimination for higher confidence genotype calling
- Tight clustering, easy analysis – perfect for difficult SNPs
- Optimized – Assays quickly and reliably established, even with difficult genomic loci
- Cost effective – Ideal for large scale genotyping projects
SensiFAST Lo-ROX Genotyping Kit
SensiFAST Hi-ROX Genotyping Kit
SensiFAST HRM Kit
SensiFAST Probe Application Note
Download the Application Note – Use of Multiplex Real-Time PCR in prenatal testing.
SensiFAST Instrument Compatibility
Use Bioline´s selection table to identify the most suitable product for your real-time PCR instrument.
SensiFAST Guide
Download the SensiFAST™ Real-Time PCR Guide with detailed product descriptions, to help you choose the correct product for your research.