TotalSeq™ Antibody Oligonucleotide Conjugates

Great advances in next generation sequencing (NGS) and droplet-based microfluidic technologies have enabled transcriptome level data generation from thousands of single cells, simultaneously, via high-throughput single-cell RNA sequencing (scRNA-seq). Recently, the 10x Genomics Chromium™ System plus Single Cell 3’ Solution have facilitated widespread adoption of scRNA-seq in biomedical research, providing hope that the method can be used to expedite the path to precision medicine. Despite the vital importance of proteins in biology, it has not previously been possible to incorporate proteomics into these high throughput scRNA-seq assays. To this end, BioLegend is proud to announce our TotalSeq™ product line, which enables the generation of both single cell transcriptomic and proteomic data simultaneously, in high throughput, catalyzing the next wave of legendary discovery in biomedical research.

Simultaneous Multiomic Data Generation: Increased power of single cell experiments by adding proteomic and transcriptomic data.
Reduced Dropouts: In contrast to mRNA, TotalSeq™- derived tags are not highly prone to dropouts, which are essentially false negative readouts.
Enhanced Cell Type Identification: This can be achieved thanks to lower dropout rate and thus, enhanced sample clustering.
Research Areas
• Personalized or Precision Medicine
• Cancer Research
• Stem Cell Research
• Basic and Applied Immunology
• Biomarker Discovery
• Characterization of New and Rare Cell Types
• Neuroimmunology
• Vaccine Research

View and order TotalSeq™ products