alpha-Synuclein Specific T Cells in Parkinson's Disease

In Parkinson's disease (PD) patients, certain MHC alleles have been found to be more prevalent than others. Sulzer et al. wanted to determine whether PD is associated with an antigen-specific T cell response to α-Synuclein, a hallmark PD protein that aggregates and forms Lewy Bodies. Sulzer produced an α-Synuclein peptide library and tested PBMCs from PD patients. They measured IFN-γ production from Th1 and CD8+ T cells, as well as IL-5 secretion from Th2 cells.

The Y39 and S129 epitope regions elicited the most immune responses from the listed MHC I and II alleles below. These epitopes are sensitive to PD-promoting mutations, and the alleles are present at higher frequencies in PD patients compared to healthy controls. This indicates that α-Synuclein in the cerebrospinal fluid could be captured by antigen presenting cells, displayed by PD-associated MHC alleles, and promote adaptive immune responses.

BioLegend offers several antibodies to detect phosphorylated and aggregated/fibrillar forms of α-Synuclein. In addition, we provide Flex-T™, a unique tetramer system that allows peptides of interest to be exchanged in the MHC groove.

α-Synuclein Antibodies

 Clone  Target Recognition
4B12/Synuclein Monomers, Fibrils
A15119B Phospho (Tyr39)
A15126D 117-122 aa
A15127A Truncated C-terminus, x-122 aa
P-syn/81A Phospho (Ser129)
Syn-F1 Fibrils
Syn-O2 Oligomers
Syn-O3 Oligomers
Syn-O4 Oligomers

α-Synuclein ELISAs