Antibody and IHC-protocol optimization service


- FFPE tissue sections
- Frozen tissue sections
- Polymer based detection
- Chromogenic visualization
- Fluorescence visualization
- Sectioning is included in the price
- Short report with images will be delivered to the client
- Protocol design service and antibody searching service

Optimization of antibodies

 Service Packet

 Description  Pricing per antibody*

Packet 1:

- Client delivered primary antibodies
- Client delivered tissues

Optimization will be performed using three different pre-treatment method and six different dilutions of antibody. Total amount of IHC slides will be 18 pcs and negative tissue controls.

350 €

Packet 2:

- Optimization of antibodies supplied by BioSiteHisto
- Optimization with tissues supplied by BioSiteHisto

Optimization will be performed using three different pre-treatment method and six different dilutions of antibody. Total amount of IHC slides will be 18 pcs and negative tissue controls.

550 - 800 €

Exact price will depend on cost of the antibody

BioSiteHisto will charge cost of the antibody from client

Packet 3:

- Optimization of mouse or rat antibodies in mouse or rat tissue
- Client delivered primary antibodiesand/or tissues
- or optimization of antibodies and tissues supplied by BioSiteHisto

Optimization will be performed using three different pre-treatment method and six different dilutions of antibody. Total amount of IHC slides will be 18 pcs and negative tissue controls.

600 - 800 €

* these prices are from prices and may vary based on project-specific requirements. Invoicing will be made in local currency.

Example of the optimization table

Dilution / 
Pretreatment

1:25 1:50 1:100 1:200 1:400 1:800
No Pretreatment            
HIER pH9            
HIER pH6