Article No
10-10011-100
GAPDH
Monoclonal Antibody to GAPDH (Clone: ABM22C5)
Antibodies Primary
Specifications
| Application | IHC, WB |
| Article No | 10-10011-100 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | ABM22C5 |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | Monoclonal Antibody to GAPDH (Clone: ABM22C5) |
| Supplier | Abeomics |
| Entrez Gene ID | 2597 |
| Gene Symbol | GAPDH |
| Immunogen | A partial length recombinant GAPDH protein (amino acids 120-320) was used as the immunogen for this antibody. |
| Isotype | IgG1 k |
| Keywords | Loading Controls, GAPDH, 2597, P04406, GAPDH, GAPD, CDABP0047, OK/SW-cl.12, ABM22C5, Monoclonal Antibody to GAPDH(Clone :ABM22C5), Anti GAPDH Antibody, GAPDH Antibody |
| Notes | GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is an enzyme best known for its role in glycolysis. However, extra-glycolytic functions of GAPDH have been described, including regulation of protein expression via RNA binding. GAPDH binds to numerous AREs (adenine-uridine rich elements) from various mRNA 3'-untranslated regions in vitro and in vivo despite its lack of a canonical RNA binding motif. GAPDH specifically catalyzes the simultaneous phosphorylation and oxidation of glyceraldehyde 3-phosphate using NAD+ (Nicotinamide Adenine Dinucleotide) as a cofactor to produce glycerate 1,3-biphosphate and NADH. In addition to its role in energy production, GAPDH has been implicated in many cellular processes including DNA repair tRNA export, membrane fusion and transport, endocytosis and nuclear membrane assembly, and cell death. |
| Alias Names | GAPDH||GAPD||CDABP0047||OK/SW-cl.12 |
| Product Type | Antibodies Primary |
| Protocol | Western blot analysis: 0.5-2 µg/ml, Immunohistochemical analysis: 5 µg/ml |
| Purification | Protein G Purified |
| Shipping Information | Blue Ice |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | human, mouse |
| Storage | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
| Substrate / Buffer | 100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| UniProt Number | P04406 |
| Product Page Updated | 2024-04-03T14:02:41.041Z |
