Article No
109-156-127
AMCA-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Human IgG + IgM (H+L) (min X Bov Sr Prot)
AMCA-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Human IgG + IgM (H+L) (min X Bov Sr Prot)
Antibodies Secondary
Specifications
| Application | IF |
| Article No | 109-156-127 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone Type | polyclonal |
| Conjugation | AMCA |
| Description | AMCA-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Human IgG + IgM (H+L) (min X Bov Sr Prot) |
| Recommended Dilution | _x000D_ 1:50 - 1:200 for most applications_x000D_ _x000D_ Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
| Supplier | Jackson ImmunoResearch |
| Format | Affinity Purified |
| Immunogen | IgG + IgM (H+L) |
| Notes | F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with both human IgG and IgM. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine serum proteins, but it may cross-react with immunoglobulins from other species. |
| Product Type | Antibodies Secondary |
| Protocol | 1:50 - 1:200 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
| Purity | AffiniPure |
| Shipping Information | RT |
| Size | 1 mg |
| Source / Host | goat |
| Species Reactivity | human |
| Storage | 4°C |
| Substrate / Buffer | 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 |
| Product Page Updated | 2023-12-29T14:15:50.354Z |
