Article No
115-627-020
Application | Labeling |
Article No | 115-627-020 |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Clone Type | polyclonal |
Conjugation | Alexa Fluor 680 |
Description | Alexa Fluor® 680-conjugated AffiniPure Fab Fragment Goat Anti-Mouse IgM, µ Chain Specific |
Recommended Dilution | Lbl 1:100 - 1:800 |
Supplier | Jackson ImmunoResearch |
Format | Affinity Purified |
Immunogen | IgM, µ chain |
Isotype | IgM |
Notes | Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa. Based on antigen-binding assay and/or ELISA, the antibody reacts with the heavy chain of mouse IgM but not with mouse IgG or the light chains of mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with IgM from other species. |
Product Type | Antibodies Secondary |
Protocol | 1:100 - 1:800 for sequential labeling applications. To complex with primary antibody in solution, use 1:1 weight ratio of Fab:primary antibody (15:1 molar ratio). Vortex and incubate for 30 minutes at room temperature prior to use. Titrate complex to optimal dilution for assay. Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Purity | AffiniPure |
Shipping Information | RT |
Size | 0.5 mg |
Source / Host | goat |
Species Reactivity | mouse |
Storage | 4°C |
Substrate / Buffer | 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 |
Product Page Updated | 2023-12-29T14:15:50.354Z |