Article No
115-627-185
Alexa Fluor® 680-conjugated AffiniPure Fab Fragment Goat Anti-Mouse IgG1, Fcγ Fragment Specific
Alexa Fluor® 680-conjugated AffiniPure Fab Fragment Goat Anti-Mouse IgG1, Fcγ Fragment Specific
Antibodies Secondary
Specifications
| Application | Labeling |
| Article No | 115-627-185 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone Type | polyclonal |
| Conjugation | Alexa Fluor 680 |
| Description | Alexa Fluor® 680-conjugated AffiniPure Fab Fragment Goat Anti-Mouse IgG1, Fcγ Fragment Specific |
| Recommended Dilution | Lbl 1:100 - 1:800 |
| Supplier | Jackson ImmunoResearch |
| Format | Affinity Purified |
| Immunogen | IgG1, Fcγ fragment |
| Isotype | IgG1 |
| Notes | Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa. Based on antigen-binding assay and/or ELISA, the antibody reacts with the Fc portion of mouse IgG1 but not with the Fab portion of mouse immunoglobulins. No antibody was detected against mouse IgM or against non-immunoglobulin serum proteins. The antibody may cross-react with other mouse IgG subclasses or with immunoglobulins from other species. |
| Product Type | Antibodies Secondary |
| Protocol | 1:100 - 1:800 for sequential labeling applications. To complex with primary antibody in solution, use 1:1 weight ratio of Fab:primary antibody (3:1 molar ratio). Vortex and incubate for 30 minutes at room temperature prior to use. Titrate complex to optimal dilution for assay. Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
| Purity | AffiniPure |
| Shipping Information | RT |
| Size | 0.5 mg |
| Source / Host | goat |
| Species Reactivity | mouse |
| Storage | 4°C |
| Substrate / Buffer | 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 |
| Product Page Updated | 2023-12-29T14:15:50.354Z |