Antibodies Primary

Vimentin (Clone 12E4)

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Vimentin (Clone 12E4)

Article No

154-20197

Application

ELISA, IP, WB

Size

100 µg

Clone

1.20E+05

Source / Host

mouse

Shipping Information

-20

Specifications

Additional Information Immunogen: Recombinant human vimentin • Host: Mouse • Species Reactivity: (+) Human • Cross Reactivity: (+) Vimentin, citrullinated vimentin • Applications: ELISA, IP, WB • MW: ~54 kDa
Application ELISA, IP, WB
Article No 154-20197
Country Availability SE, FI, DK, NO, IS, EE, LV, LT, RU
Clone 1.20E+05
Description Vimentin (Clone 12E4)
Supplier Cayman Chemical
Immunogen Recombinant human vimentin
Isotype IgG2a
Notes Vimentin is a cytoskeleton intermediate filament protein.{42183} It is composed of monomers that each contain a central α-helix rod domain, which facilitates formation of a coiled-coil dimer required for vimentin filament assembly, as well as N-terminal head and C-terminal tail domains.{42183,42184} It is expressed in mesenchymal stem cells and cells of mesenchymal origin, including leukocytes, endothelial cells, and smooth muscle cells.{60331} Vimentin is attached to nuclei, endoplasmic reticulum, and mitochondria, and has a role in positioning organelles in the cytosol.{42184} It regulates glial morphology, facilitates motility and directional migration of fibroblasts, and is critical to mechanotransduction of shear stress and maintenance of vascular endothelial integrity.{42183} Vimentin controls transport of LDL-derived cholesterol from lysosomes to esterification sites.{42185} It is an aggresome component, forming a cage-like structure around aggregated, undegraded proteins at the microtubule organizing center.{42186} Vimentin is subject to citrullination under high calcium concentrations, which can occur during macrophage apoptosis, and citrullinated vimentin has been shown to have a role in the production of anti-citrullinated protein antibodies (ACPAs).{31883,31884} ACPAs against citrullinated proteins, such as vimentin, are considered to be highly specific markers for rheumatoid arthritis and other autoimmune diseases.{31883} Cayman's Vimentin Monoclonal Antibody (Clone 12E4) can be used for ELISA, immunoprecipitation (IP), and Western blot (WB) applications. The antibody recognizes vimentin at approximately 54 kDa from human samples.
Alias Names VIM
Product Type Antibodies Primary
Shipping Information -20
Size 100 µg
Source / Host mouse
Storage -20
Substrate / Buffer 100 μg of Protein G-purified IgG2a
Product Page Updated 2021-11-09T07:05:51.039Z

Shipping info

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