Article No
154-30589
Proteins & Peptides
SARS-CoV-2 nsp13 Helicase (E. coli expressed)
SARS-CoV-2 nsp13 Helicase (E. coli expressed)
Specifications
| Additional Information | Source: Active recombinant nsp13 helicase expressed in E. coli • Amino acids: 5325-5925 • MW: 69.1 kDa |
| Article No | 154-30589 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, RU |
| Description | SARS-CoV-2 nsp13 Helicase (E. coli expressed) |
| Supplier | Cayman Chemical |
| Notes | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53462,53459,53461,55257,55258} The SARS-CoV-2 genome contains approximately 30 kilobases and 14 open reading frames (ORFs) that encode four structural proteins: spike, envelope, membrane, and nucleocapsid, as well as 16 non-structural proteins and 9 accessory factors.{57176} SARS-CoV-2 nsp13 helicase is a superfamily 1B helicase and component of replicase polyprotein 1a (PP1a), which is encoded by ORF1a in the viral genome. It is composed of two canonical RecA-like ATPase domains, 1A and 2A, and a zinc-binding domain, stalk region, and 1B domain.{57175,57181} SARS-CoV-2 nsp13 functions as both an RNA helicase and a nucleoside triphosphate hydrolase (NTPase), unwinding RNA in an NTP-dependent manner.{57177} It inhibits IFN-β reporter gene activation induced by the RIG-I 2CARD domain in 293FT cells, indicating a role for nsp13 helicase in suppression of the host immune response.{57182} Cayman's SARS-CoV-2 nsp13 Helicase (E. coli expressed) protein can be used for enzyme assays and Western blot (WB) applications. |
| Alias Names | 2019-nCOV Helicase|COVID-19 Helicase|NSP13|SARS-CoV2 Helicase|SARS-CoV-2 Helicase, COVID19 Helicase |
| Product Type | Proteins & Peptides |
| Purity | ≥80% estimated by SDS-PAGE |
| Shipping Information | -80 |
| Size | 10 µg |
| Storage | -80 |
| Substrate / Buffer | 50 mM Tris-HCl, pH 7.5, with 5 µM zinc chloride, 1 mM DTT, 1 mM EDTA, and 10% glycerol |
| Product Page Updated | 2021-02-17T10:48:22.248Z |
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