Article No
154-31816
Proteins & Peptides
SARS-CoV-2 nsp16 Methyltransferase
SARS-CoV-2 nsp16 Methyltransferase
Specifications
| Additional Information | Source: Recombinant C-terminal His-tagged SARS-CoV-2 nsp16 methyltransferase expressed in E. coli • Amino acids: 6799-7096 • MW: 33.5 kDa |
| Article No | 154-31816 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, RU |
| Description | SARS-CoV-2 nsp16 Methyltransferase |
| Supplier | Cayman Chemical |
| Notes | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53462,53459,53461,55257,55258} The SARS-CoV-2 genome contains approximately 30 kilobases and 14 open reading frames (ORFs) that encode four structural proteins: spike, envelope, membrane, and nucleocapsid, as well as 16 non-structural proteins and 9 accessory factors.{57176} SARS-CoV-2 nsp16 methyltransferase is a 2'-O-methyltransferase (2'-O-MTase) and has a role in 5'-end capping of viral mRNAs.{52793,52794} In coronaviruses, nsp16 forms a complex with nsp10 to methylate nascent mRNAs at the ribose 2'-O position, creating a Cap-1 structure that facilitates increased translation of viral mRNAs and reduced innate immune recognition by the host cell.{52794,52795} Deficiency of nsp16 in the related virus SARS-CoV reduces viral RNA synthesis by approximately 10-fold in vitro, and mutations in the nsp16 KDKE catalytic tetrad in mouse-adapted SARS-CoV attenuate the virus in vivo.{52794,52796} An nsp10-derived peptide inhibitor of the nsp16/nsp10 complex increases survival in a mouse model of infection with the coronavirus mouse hepatitis virus (MHV).{52795} Cayman's SARS-CoV-2 nsp16 Methyltransferase protein consists of 299 amino acids and has a calculated molecular weight of 33.46 kDa. |
| Alias Names | 2019-nCoV nsp16|2'-O-MTase, ME, nsp16, 2'-O-methyltransferase, SARS-CoV-2 nsp16|SARS-CoV2 nsp16|SCoV2-nsp16 |
| Product Type | Proteins & Peptides |
| Purity | ≥85% as determined by SDS-PAGE |
| Shipping Information | -20 |
| Size | 100 µg |
| Storage | -20 |
| Substrate / Buffer | sterile 10 mM Tris, pH 7.4, with 250 mM NaCl, and 50% glycerol pH 7.4 |
| Product Page Updated | 2021-02-17T10:48:22.248Z |
Shipping info
The delivery time for this item is approximately 8-10 business days.
If other deviations occur, this will be noted on the order confirmation or communicated via email.
Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.