Proteins & Peptides

SARS-CoV-2 nsp16 Methyltransferase

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SARS-CoV-2 nsp16 Methyltransferase

Article No

154-31816

Size

100 µg

Shipping Information

-20

Documentation

Specifications

Additional Information Source: Recombinant C-terminal His-tagged SARS-CoV-2 nsp16 methyltransferase expressed in E. coli • Amino acids: 6799-7096 • MW: 33.5 kDa
Article No 154-31816
Country Availability SE, FI, DK, NO, IS, EE, LV, LT, RU
Description SARS-CoV-2 nsp16 Methyltransferase
Supplier Cayman Chemical
Notes Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53462,53459,53461,55257,55258} The SARS-CoV-2 genome contains approximately 30 kilobases and 14 open reading frames (ORFs) that encode four structural proteins: spike, envelope, membrane, and nucleocapsid, as well as 16 non-structural proteins and 9 accessory factors.{57176} SARS-CoV-2 nsp16 methyltransferase is a 2'-O-methyltransferase (2'-O-MTase) and has a role in 5'-end capping of viral mRNAs.{52793,52794} In coronaviruses, nsp16 forms a complex with nsp10 to methylate nascent mRNAs at the ribose 2'-O position, creating a Cap-1 structure that facilitates increased translation of viral mRNAs and reduced innate immune recognition by the host cell.{52794,52795} Deficiency of nsp16 in the related virus SARS-CoV reduces viral RNA synthesis by approximately 10-fold in vitro, and mutations in the nsp16 KDKE catalytic tetrad in mouse-adapted SARS-CoV attenuate the virus in vivo.{52794,52796} An nsp10-derived peptide inhibitor of the nsp16/nsp10 complex increases survival in a mouse model of infection with the coronavirus mouse hepatitis virus (MHV).{52795} Cayman's SARS-CoV-2 nsp16 Methyltransferase protein consists of 299 amino acids and has a calculated molecular weight of 33.46 kDa.
Alias Names 2019-nCoV nsp16|2'-O-MTase, ME, nsp16, 2'-O-methyltransferase, SARS-CoV-2 nsp16|SARS-CoV2 nsp16|SCoV2-nsp16
Product Type Proteins & Peptides
Purity ≥85% as determined by SDS-PAGE
Shipping Information -20
Size 100 µg
Storage -20
Substrate / Buffer sterile 10 mM Tris, pH 7.4, with 250 mM NaCl, and 50% glycerol pH 7.4
Product Page Updated 2021-02-17T10:48:22.248Z

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