Lysates

3T3 (Balb) Lysate

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3T3 (Balb) Lysate

Article No

155-1212

Application

WB

Species Reactivity

mouse

Size

0.1 mg

Source / Host

mouse

Shipping Information

blue ice or RT

Documentation

Suggested protocols

Specifications

Application WB
Article No 155-1212
Country Availability SE, FI, DK, NO, IS, EE, LV, LT, FO, GL, RU
Concentration 2 mg/mL
Description 3T3 (Balb) Lysate
Supplier ProSci
Format Liquid
Notes 3T3 (Balb) lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The 3T3 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Previous Article No 155-1212-0.1
Product Type Lysates
Shipping Information blue ice or RT
Size 0.1 mg
Source / Host mouse
Species Reactivity mouse
Storage Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Substrate / Buffer 3T3 (Balb) lysate is supplied in SDS sample buffer containing 50 mM DTT.
Tissue Type BALB/3T3 clone A31 (Mouse embryo)
Product Page Updated 2021-01-13T13:08:44.986Z

Shipping info

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