3T3 (Balb) Lysate

3T3 (Balb) Lysate

Article No




Species Reactivity



0.1 mg

Source / Host


Shipping Information

blue ice or RT


Suggested protocols


Application WB
Article No 155-1212
Country Availability SE, FI, DK, NO, IS, EE, LV, LT, FO, GL, RU
Concentration 2 mg/mL
Description 3T3 (Balb) Lysate
Supplier ProSci
Format Liquid
Notes 3T3 (Balb) lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The 3T3 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Previous Article No 155-1212-0.1
Product Type Lysates
Shipping Information blue ice or RT
Size 0.1 mg
Source / Host mouse
Species Reactivity mouse
Storage Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Substrate / Buffer 3T3 (Balb) lysate is supplied in SDS sample buffer containing 50 mM DTT.
Tissue Type BALB/3T3 clone A31 (Mouse embryo)
Product Page Updated 2021-01-13T13:08:44.986Z

Shipping info

The delivery time for this item is approximately 5-8 business days. 
If other deviations occur, this will be noted on the order confirmation or communicated via email.
Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.