Article No
155-33-218
Specifications
| NCBI Number | 7157 |
| Application | FC, IF, IHC, WB |
| Article No | 155-33-218 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone | DO-7 |
| Clone Type | monoclonal |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Description | p53 Antibody [DO-7] |
| Supplier | ProSci |
| Entrez Gene ID | 7157 |
| Format | Liquid |
| Gene Symbol | TP53 |
| Immunogen | Recombinant human wild type p53 expressed in E. coli was used as the immunogen for this antibody. Its epitope maps within the N-terminus portion (aa 20-25) of the p53 oncoprotein. |
| Isotype | IgG2b k |
| Notes | This antibody is specific for a 53kDa protein, which is identified as p53 suppressor gene product. The large number of prolines in its amino acid sequence causes p53 to migrate slowly in SDS-PAGE, resulting in the amino acid content-estimated 43 kDa protein appearing larger than expected. DO-7 reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intra-tubular germ cell neoplasia. |
| Alias Names | Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53 |
| Previous Article No | 155-33-218-100 |
| Product Type | Antibodies Primary |
| Protocol | Flow Cytometry: 0.5-1 ug/million cells IF: 0.5-1 ug/ml WB: 0.5-1.0 ug/ml IHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the p53 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
| Purification | Protein G Purified |
| Research area | Cell Proliferation |
| Shipping Information | BLUE ICE |
| Size | 100 ug |
| Source / Host | mouse |
| Species Reactivity | cow/bovine, human, monkey |
| Storage | 4°C |
| Substrate / Buffer | PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
| UniProt Number | P04637 |
| Product Page Updated | 2024-01-03T13:34:25.701Z |
