Article No
155-XR-9740
Goat Anti-Mouse IgG (H&L) Antibody (biotin)
Goat Anti-Mouse IgG (H&L) Antibody (biotin)
Antibodies Secondary
Specifications
| Application | ELISA |
| Article No | 155-XR-9740 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone Type | polyclonal |
| Conjugation | Biotin |
| Description | Goat Anti-Mouse IgG (H&L) Antibody (biotin) |
| Supplier | ProSci |
| Format | Lyophilized |
| Immunogen | Mouse IgG - whole molecule |
| Isotype | IgG |
| Previous Article No | 155-XR-9740-2 |
| Product Type | Antibodies Secondary |
| Protocol | Biotin Conjugated Affinity Purified anti-Mouse IgG is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:200,000 of the reconstitution concentration is suggested for this product. (Optimal dilution should be determined by user). |
| Purification | Affinity Purified |
| Shipping Information | RT |
| Size | 2 mg |
| Source / Host | goat |
| Species Reactivity | mouse |
| Storage | Biotin Conjugated Affinity Purified anti-Mouse IgG can be stored vial at 4 °C prior to restoration. For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. |
| Substrate / Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% (w/v) Sodium Azide; pH 7.2; 10 mg/mL Bovine Serum Albumin |
| Technical Specifications | Biotin Conjugated Affinity Purified anti-Mouse IgG is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:200,000 of the reconstitution concentration is suggested for this product. (Optimal dilution should be determined by user). |
| Product Page Updated | 2024-01-25T14:36:17.736Z |
