Article No
173-24274
5-HIAA (5-Hydroxyindoleacetic Acid) Antibody
5-HIAA (5-Hydroxyindoleacetic Acid) Antibody
Antibodies Primary
Specifications
| Application | ICC, EM, IF, IHC |
| Article No | 173-24274 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone Type | polyclonal |
| Conjugation | Unconjugated |
| Description | 5-HIAA (5-Hydroxyindoleacetic Acid) Antibody |
| Supplier | ImmunoStar |
| Format | Whole Serum |
| Immunogen | 5-HIAA |
| Notes | The 5-HIAA antibody was raised to 5-HIAA coupled to BSA with paraformaldehyde. The antibody produces moderate labeling of raphe neurons in normal rat. In rats whose serotonergic system has been activated, staining intensity is increased to a maximum label. Recommended dilutions of the antiserum are 1/200-1/400 for indirect immunofluorescence and 1/4000-1/8000 for biotin-streptavidin/HRP technique. The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Schipper and Tilders, 1983). Results showed that the 5-HIAA antibody dose dependently stained 5-HIAA but did not stain any concentration of 5-HT or 5-HTP. The antiserum was also tested by pre-adsorption at 25 µg/mL with various BSA conjugates. While pre-adsorption with 5-HIAA conjugate completely eliminates immunolabeling, pre-adsorption with conjugates of 5-HT,5-HTP and dopamine had no effect on staining intensity or distribution of stain. |
| Previous Article No | 173-24274, 24274 |
| Product Type | Antibodies Primary |
| Research area | Metabolism |
| Size | 100 µl |
| Source / Host | rabbit |
| Species Reactivity | rat |
| Product Page Updated | 2024-01-03T12:30:51.564Z |
