Article No
20-1003-50
Specifications
| Application | IHC, IP, WB |
| Article No | 20-1003-50 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone Type | polyclonal |
| Conjugation | Unconjugated |
| Description | Polyclonal antibody to APAF-1 |
| Supplier | Abeomics |
| Entrez Gene ID | 317 |
| Gene Symbol | APAF1 |
| Immunogen | A synthetic peptide of APAF-1 protein (amino acids 451-468 TQFQRYHQPHTLSPDQED) was used as the immunogen for this antibody |
| Isotype | IgG |
| Notes | Apaf 1 (apoptosis protease-activating factor-1) is a key regulator of the mitochondrial apoptotic pathway, being the central element of the multimeric apoptosome. The apoptosome consists of cytochrome c, procaspase-9 and seven Apaf 1 monomers, and is considered to be core apoptotic machinery that executes mitochondria-dependent apoptosis. Cytochrome c, normally compartmentalized in the mitochorndria, is released into the cytoplasm following apoptotic stimuli. Apaf 1 binds cytochrome c in the cytoplasm and in the presence of dATP/ATP forms the apoptosome. The apoptosome binds procaspase-9 and promotes its autocatalytic activation. Active caspase-9, in turn, activates downstream caspases including 3, 6, and 7 contributing to the proteolytic caspase activation cascade which leads to cell death. |
| Alias Names | APAF1||KIAA0413 |
| Product Type | Antibodies Primary |
| Protocol | WB: 1:1000-1:2000, IHC (paraffin): 1:1000-1:5000, IHC (frozen): Users should optimize, IP: 1:50-1:200 |
| Research area | Cell Death |
| Shipping Information | Blue Ice |
| Size | 50 µl |
| Source / Host | rabbit |
| Species Reactivity | human |
| Storage | Store the antibody at 4⁰C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
| Substrate / Buffer | 50 µl sera |
| UniProt Number | O14727 |
| Product Page Updated | 2024-04-03T14:02:41.041Z |
