Article No
209-6CD889
Suggested protocols
Specifications
| Additional Information | Heat lysate to 95C for 5 minutes and rapidly cooling before use. |
| Application | WB |
| Article No | 209-6CD889 |
| Country Availability | all |
| Concentration | 2mg/ml (determined by BCA assay) |
| Description | 293T |
| Supplier | Nordic BioSite |
| Notes | 293T cells are a human embryonic kidney (HEK) cell line that expresses the large T antigen. The large T antigen is commonly used for transfection assays and is required for transient transfection of plasmids with an SV40 origin of replication. 293T cells are able to grow well in reduced serum medium or in medium lacking calcium ions. In addition, 293T cells tend to grow at a faster rate and to a higher cell density than the parent 293 cell line, resulting in increased amounts of endogenously expressed protein. |
| Product Type | Lysates |
| Protocol | Heat lysate to 95C for 5 minutes and rapidly cooling before use. |
| Size | 200ug |
| Species Reactivity | human |
| Storage | Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles. |
| Substrate / Buffer | Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl pH6.8, 0.4% SDS, 5% Glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue) |
| Technical Specifications | Western Blot analyzed by both GAPDH and Beta tubulin antibody |
| Product Page Updated | 2020-03-03T13:42:17.283Z |
Shipping info
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