Lysates

293T

NEW
293T

Article No

209-DF521C

Application

WB

Species Reactivity

human

Size

100ug

Suggested protocols

Specifications

Additional Information Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Application WB
Article No 209-DF521C
Country Availability all
Concentration 2mg/ml (determined by BCA assay)
Description 293T
Supplier Nordic BioSite
Notes 293T cells are a human embryonic kidney (HEK) cell line that expresses the large T antigen. The large T antigen is commonly used for transfection assays and is required for transient transfection of plasmids with an SV40 origin of replication. 293T cells are able to grow well in reduced serum medium or in medium lacking calcium ions. In addition, 293T cells tend to grow at a faster rate and to a higher cell density than the parent 293 cell line, resulting in increased amounts of endogenously expressed protein.
Product Type Lysates
Protocol Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Size 100ug
Species Reactivity human
Storage Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Substrate / Buffer Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl pH6.8, 0.4% SDS, 5% Glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Technical Specifications Western Blot analyzed by both GAPDH and Beta tubulin antibody
Product Page Updated 2020-03-03T13:42:17.283Z

Shipping info

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