Article No
212-585-082
Alexa Fluor® 594-conjugated AffiniPure Mouse Anti-Rat IgG (H+L) (min X Ms Sr Prot)
Alexa Fluor® 594-conjugated AffiniPure Mouse Anti-Rat IgG (H+L) (min X Ms Sr Prot)
Antibodies Secondary
Specifications
| Application | IF, FC |
| Article No | 212-585-082 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone Type | polyclonal |
| Conjugation | Alexa Fluor 594 |
| Description | Alexa Fluor® 594-conjugated AffiniPure Mouse Anti-Rat IgG (H+L) (min X Ms Sr Prot) |
| Recommended Dilution | 1:100 - 1:800 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
| Supplier | Jackson ImmunoResearch |
| Format | Affinity Purified |
| Immunogen | IgG (H+L) |
| Isotype | IgG |
| Notes | Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody exhibits inherent minimal cross-reaction to mouse serum proteins and has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with mouse serum proteins. The antibody may cross-react with immunoglobulins from other species. |
| Product Type | Antibodies Secondary |
| Protocol | 1:100 - 1:800 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
| Purity | AffiniPure |
| Shipping Information | RT |
| Size | 1 mg |
| Source / Host | mouse |
| Species Reactivity | rat |
| Storage | 4°C |
| Substrate / Buffer | 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 |
| Product Page Updated | 2023-12-29T14:15:50.354Z |
