Article No
246-GAGp/C3c/Bio
Goat anti Guinea Pig C3c, conjugated with Biotin
Goat anti Guinea Pig C3c, conjugated with Biotin
Antibodies Primary
Specifications
| Application | ELISA, IF |
| Article No | 246-GAGp/C3c/Bio |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | Polyclonal |
| Clone Type | polyclonal |
| Conjugation | Biotin |
| Cross Reactivity | The antiSerum does not cross-react with any other component of Guinea Pig plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail. |
| Description | Goat anti Guinea Pig C3c, conjugated with Biotin |
| Supplier | Nordic - MUbio |
| Format | Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity. |
| Notes | In immunoelectrophoresis against fresh guinea pig serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of guinea pig serum or plasma. The biotin immunoconjugate to guinea pig C3c is used to determine the presence and pattern of C3 in tissue lesions using immunohistochemical staining techniques. Locally deposited immune complexes in tissue usually contain complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and he extent of tissue destruction. In ELISA and Western blotting to identify guinea pig C3c in serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Previous Article No | 246-GAGp/C3c/Bio |
| Product Type | Antibodies Primary |
| Protocol | ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting. |
| Size | 1 ml |
| Source / Host | goat |
| Species Reactivity | guinea pig |
| Storage | The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below - 20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. |
| Substrate / Buffer | Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity. |
| Technical Specifications | Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. |
| Product Page Updated | 2024-01-31T10:35:40.347Z |
