Article No
246-MAHu/IgAc/PO
Mouse anti Human IgA1+IgA2 , conjugated with Horseradish peroxidase
Mouse anti Human IgA1+IgA2 , conjugated with Horseradish peroxidase
Antibodies Secondary
Specifications
| Application | WB, ELISA |
| Article No | 246-MAHu/IgAc/PO |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | NI 69 (A89-034) and NI 184 (A89-035) |
| Clone Type | monoclonal |
| Conjugation | HRP |
| Cross Reactivity | The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species. |
| Description | Mouse anti Human IgA1+IgA2 , conjugated with Horseradish peroxidase |
| Supplier | Nordic - MUbio |
| Format | Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
| Isotype | IgG1 |
| Notes | The reactivity of the antiserum is restricted to determinants on the CH2 domain of IgA. It reacts with both subclasses of IgA as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103- 148). To identify the presence of IgA in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50; in ELISA from 1:50 upwards; in Western blotting from 1:100 upwards. |
| Previous Article No | 246-MAHu/IgAc/PO |
| Product Type | Antibodies Secondary |
| Protocol | ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting. |
| Research area | Immunology |
| Size | 0.2 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day. |
| Substrate / Buffer | Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
| Technical Specifications | Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. |
| Product Page Updated | 2024-01-31T10:35:40.347Z |
