Article No
246-MAHu/IgG1/FITC
Mouse anti Human IgG1 (Fc subclass specific), conjugated with FITC
Mouse anti Human IgG1 (Fc subclass specific), conjugated with FITC
Antibodies Secondary
Specifications
| Application | FC, IF |
| Article No | 246-MAHu/IgG1/FITC |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | NI 132 (HP 6186) |
| Clone Type | monoclonal |
| Conjugation | FITC |
| Cross Reactivity | The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species. |
| Description | Mouse anti Human IgG1 (Fc subclass specific), conjugated with FITC |
| Supplier | Nordic - MUbio |
| Format | Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
| Isotype | IgG1 |
| Notes | The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc part of the IgG1 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50; in ELISA from 1:200 upwards; in Western blotting from 1:400 upwards. |
| Previous Article No | 246-MAHu/IgG1/FITC |
| Product Type | Antibodies Secondary |
| Protocol | ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. |
| Research area | Immunology |
| Size | 0.2 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day. |
| Substrate / Buffer | Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
| Technical Specifications | Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. |
| Product Page Updated | 2024-01-31T10:35:40.347Z |
