Article No
246-MAHu/IgG2/Bio
Application | ELISA, IF |
Article No | 246-MAHu/IgG2/Bio |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Clone | NI 6014 (HP 6014) |
Clone Type | monoclonal |
Conjugation | Biotin |
Cross Reactivity | The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species. |
Description | Mouse anti Human IgG2 (Fab subclass specific), conjugated with Biotin |
Supplier | Nordic - MUbio |
Format | Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml |
Isotype | IgG1 |
Notes | The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab portion of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 250; in ELISA from 1:100 upwards; in Western blotting from 1:500 upwards. |
Previous Article No | 246-MAHu/IgG2/Bio |
Product Type | Antibodies Secondary |
Protocol | ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting. |
Research area | Immunology |
Size | 0.2 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day. |
Substrate / Buffer | Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml |
Technical Specifications | Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 4.7. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. |
Product Page Updated | 2024-01-31T10:35:40.347Z |