Article No
246-MAHu/IgGc/PO
Mouse anti Human IgG (class specific), conjugated with Horseradish peroxidase
Mouse anti Human IgG (class specific), conjugated with Horseradish peroxidase
Antibodies Secondary
Specifications
| Application | WB, ELISA |
| Article No | 246-MAHu/IgGc/PO |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | NI 335 and NI 343 |
| Clone Type | monoclonal |
| Conjugation | HRP |
| Cross Reactivity | The antibody does not react with any other component of the Human immunoglobulin system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species. |
| Description | Mouse anti Human IgG (class specific), conjugated with Horseradish peroxidase |
| Supplier | Nordic - MUbio |
| Format | Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
| Isotype | IgG1 |
| Notes | The reactivity of the this monoclonal antibody is restricted to isotype specific determinants on the Fc part of IgG. The antiserum reacts with the 4 subclasses of human IgG, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic immunoglobulins. To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:500; in ELISA from 1:1000 upwards; in Western blotting from 1:2000 upwards. |
| Previous Article No | 246-MAHu/IgGc/PO |
| Product Type | Antibodies Secondary |
| Protocol | ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting. |
| Research area | Immunology |
| Size | 0.2 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day. |
| Substrate / Buffer | Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
| Technical Specifications | Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. |
| Product Page Updated | 2024-01-31T10:35:40.347Z |
