Article No
246-MAHu/SC/PO
Application | Dot blot, ELISA, Blotting, ICC, IHC |
Article No | 246-MAHu/SC/PO |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Clone | NI 194-4 (A89-039) |
Clone Type | monoclonal |
Conjugation | HRP |
Cross Reactivity | The antiSerum does not react with any other component of the Human milk or plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species. |
Description | Mouse anti Human secretory component (free and bound), conjugated with Horseradish peroxidase |
Supplier | Nordic - MUbio |
Format | Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
Isotype | IgG1 |
Notes | The reactivity of the antiserum is restricted to free and bound secretory component as tested in indirect binding immunoblotting and indirect immunoperoxidase staining. To identify the presence of free or bound secretory component in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards; in ELISA from 1:50 upwards; in Western blotting from 1:100 upwards. These data should be interpreted as general recommendations only. |
Previous Article No | 246-MAHu/SC/PO |
Product Type | Antibodies Secondary |
Protocol | ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting. |
Research area | Immunology |
Size | 0.2 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day. |
Substrate / Buffer | Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
Technical Specifications | Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute t he lyophilized product by adding 0.5 ml sterile distilled water. |
UniProt Number | P01833 |
Product Page Updated | 2024-01-31T10:35:40.347Z |