Article No
289-MM-0161-P
Nucleobindin
Nucleobindin Rabbit Polyclonal Antibody
Antibodies Primary
Specifications
| Application | WB, EM, ICC, IF, IP, ELISA |
| Article No | 289-MM-0161-P |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | --- |
| Clone Type | polyclonal |
| Conjugation | Unconjugated |
| Description | Nucleobindin Rabbit Polyclonal Antibody |
| Recommended Dilution | If reconstituted with deionized water in 100 µg: WB: 1:1000; IF: 1:150; EM-ICC: 1:50. Optimal dilution has to be determined by the user. |
| Supplier | MediMabs |
| Format | Lyophilized immunogen affinity purified in PBS pH7.4 |
| Immunogen | Full length recombinant human nucleobindin protein. |
| Notes | Nucleobindin 1 is a 461 amino acids ubiquitous protein with an N-terminal of 25 amino acids signal peptide responsible for its initial localization to the endoplasmic reticulum (ER). It has also been detected in the cytoplasm and in various subcellular structures such as the nucleus, mitochondria and the Golgi apparatus. Nucleobindin plays a role in a number of cellullar processes such as intracellular signaling, inflammation, autoimmunity, osteogenesis and cancer. |
| Alias Names | NUCB1, NUC, CALNUC |
| Product Type | Antibodies Primary |
| Size | 100 µg |
| Source / Host | rabbit |
| Species Reactivity | human, mouse |
| Storage | Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay. |
| Substrate / Buffer | Lyophilized immunogen affinity purified in PBS pH7.4 |
| Technical Specifications | The antibody recognizes nucleobindin 1 (NucB1) |
| Product Page Updated | 2024-01-02T11:24:51.583Z |
