Article No
300-055-240
Alkaline Phosphatase-conjugated AffiniPure Rabbit Anti-His Tag
Alkaline Phosphatase-conjugated AffiniPure Rabbit Anti-His Tag
Antibodies Secondary
Specifications
| Article No | 300-055-240 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone Type | polyclonal |
| Conjugation | AP |
| Description | Alkaline Phosphatase-conjugated AffiniPure Rabbit Anti-His Tag |
| Recommended Dilution | ICC 25-50 ug / ml, IHC 25-50 ug / ml |
| Supplier | Jackson ImmunoResearch |
| Immunogen | His Tag |
| Notes | Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on antigen-binding assay and/or ELISA, the antibody reacts with proteins containing N-terminal or C-terminal His Tags (His6-His10). |
| Product Type | Antibodies Secondary |
| Protocol | ELISA:- 1:5,000-1:50,000 Western Blot:- 1:5,000-1:50,000 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
| Purity | AffiniPure |
| Shipping Information | RT |
| Size | 0.1 ml |
| Source / Host | rabbit |
| Species Reactivity | synthetic |
| Storage | 4°C |
| Product Page Updated | 2023-12-29T14:15:50.354Z |