Article No
303-0021
Mouse anti Human CD2
Mouse anti Human CD2
Antibodies Primary
Specifications
| Application | WB, ELISA |
| Article No | 303-0021 |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | T6.3 |
| Clone Type | monoclonal |
| Concentration | See vial for concentration |
| Conjugation | Unconjugated |
| Description | Mouse anti Human CD2 |
| Supplier | Exalpha Biologicals |
| Immunogen | Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T Lymphocytes activated by mixed lymphocyte culture. |
| Isotype | IgG2a |
| Notes | Mouse anti human CD2 can be used for identification of human T cells and a subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen. |
| Alias Names | T-cell surface antigen CD2; CD2 cytoplasmic domain-binding protein |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry:_x000D_ PBMC: Add 10 µl of antibody to 1 million PBMCs in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8oC. Wash twice with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. _x000D_ WHOLE BLOOD: Add10 µl of antibody to 100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. _x000D_ See instrument manufacturer’s instructions for immunofluorescence analysis with a flow cytometer or microscope. |
| Purification | Protein A/G Chromatography |
| Shipping Information | Ship at ambient temperature. |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Stability | See expiration date on vial |
| Storage | Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles |
| Substrate / Buffer | Provided as solution in phosphate buffered saline with 0.08% sodium azide |
| Technical Specifications | PBMC: Add 10 μl of MAB/10 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8oC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. . See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. |
| UniProt Number | P06729 (Human) |
| Product Page Updated | 2023-12-29T14:25:47.027Z |
