Article No
303-0954
Mouse anti Human FAS/APO-1 (CD95), conjugated with PE
Mouse anti Human FAS/APO-1 (CD95), conjugated with PE
Antibodies Primary
Specifications
| Application | WB, ELISA |
| Article No | 303-0954 |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | 3.22 |
| Clone Type | monoclonal |
| Concentration | Titered for flow cytometry |
| Conjugation | RPE |
| Description | Mouse anti Human FAS/APO-1 (CD95), conjugated with PE |
| Supplier | Exalpha Biologicals |
| Immunogen | Full length CD95 protein |
| Isotype | IgG1 |
| Notes | Activation of either the 55-kD tumor necrosis factor receptor (TNF-R1) or CD95 (Fas/Apo-1) causes apoptosis of cells and liver failure in mice, and has been associated with human liver disorders. The aim of this study was first to clarify the association between CD95 activation, hepatocyte apoptosis, and fulminant liver failure. Next, we investigated whether TNF-R1 and CD95 operate independently of each other in the induction of hepatocyte apoptosis. |
| Alias Names | hfas -PE, Anti human fas/Apo1 RPE CD95 |
| Product Type | Antibodies Primary |
| Protocol | PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. |
| Purification | Protein A/G Chromatography |
| Research area | Cell Death |
| Shipping Information | Room Temperature |
| Size | 100 Tests |
| Source / Host | mouse |
| Species Reactivity | human |
| Stability | See expiration date on vial |
| Storage | Product should be stored at 4-8ºC. DO NOT FREEZE |
| Substrate / Buffer | Provided as solution in phosphate buffered saline with 0.08% sodium azide |
| Technical Specifications | PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. |
| UniProt Number | P25445 (Human) |
| Product Page Updated | 2023-12-29T14:25:47.027Z |
