Article No
303-A110P
Application | WB, IP, IHC |
Article No | 303-A110P |
Country Availability | SE, FI, DK, NO, EE, LV, LT |
Clone Type | polyclonal |
Concentration | See vial for concentration |
Conjugation | Unconjugated |
Description | Sheep anti Human bcl-xl |
Supplier | Exalpha Biologicals |
Immunogen | A synthetic peptide corresponding to amino acids 3 to 14 of the human bcl-xl sequence |
Isotype | IgG |
Notes | bcl-x is a bcl-2-related gene that can function as a regulator of programmed cell death (apoptosis) independent of bcl-2. Alternative splicing results in two distinct bcl-x mRNAs. The larger mRNA gives rise to a protein product, bcl-xl, which is similar in size and predicted structure to bcl-2 (1). The smaller mRNA gives rise to bcl-xS. bcl-x immunoreactivity has been detected in a wide variety of cell types and the protein is typically present in the cytosol in association with the mitochondrial periphery, a property shared with bcl-2 however membrane bound forms of bcl-x have been demonstrated in thymocytes (2-4). Following the induction of apoptosis all of the bcl-x protein shifts to the membrane form (2). Of the two isoforms of bcl-x, the long (bcl-xl) is the most abundant mRNA species expressed in embryonic and adult tissues and most likely differs from bcl-2 in its regulatory activity on cell differentiation through controlled tissue specific expression (1,3). Like its homolog bcl-2, bcl-x undergoes phosphorylation, a modification that requires that a specific 60 amino acid loop region be intact, which in turn appears to regulate activity (5,6). Structurally, based on 3D-structure analysis, bcl-x forms pH sensitive cation-selective ion channels in membranes a property shared with the pore forming domains of several bacterial toxins (7). Bcl-xl has been shown to modify the cellÕs response to oxidants, to participate in resistance to chemotherapeutic agents and radiation, and to play a key role in the development of the developing CNS (8-10). |
Alias Names | hbcl-xl, sheep anti human bcl-xl, bclxl, bcl xl, bclx, bcl2L1, BCL2L |
Product Type | Antibodies Primary |
Protocol | Application: Western Blot at 1-5 ug/ml. Positive control MCF-7 cells. |
Purification | Ammonium Sulfate Precipitation |
Research Area | Cell Death |
Shipping Information | room temperature |
Size | 100 µg |
Source / Host | sheep/ovine |
Species Reactivity | human |
Stability | See expiration date on vial |
Storage | Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles |
Substrate / Buffer | Provided as solution in phosphate buffered saline with 0.08% sodium azide |
Technical Specifications | Application: Western Blot at 1-5 ug/ml. Positive control MCF-7 cells. |
UniProt Number | Q07817 (Human) |
Product Page Updated | 2023-12-29T14:25:47.027Z |