Article No
303-CK114
Specifications
| Application | ELISA, RIA |
| Article No | 303-CK114 |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | LL002 |
| Clone Type | monoclonal |
| Concentration | 40 µg/ml |
| Control Tissue | Bronchial epithelium |
| Description | Cytokeratin 14 |
| Supplier | Exalpha Biologicals |
| Immunogen | C-terminal 15-AA peptide of cytokeratin 14 conjugated to thyroglobulin |
| Isotype | IgG3 |
| Notes | Cytokeratin 14 (CK14) belongs to the acid subfamily of CKs (Type I) and is characteristic of stratifying epithelia (trachea, bronchi, cervix, epidermis, sweat glands). The antibody to CK14 is helpful to distinguish between stratifying epithelia and simple epithelia, which don't express this type of cytokeratins. Tumours of squamous cell origin will be stained with this antibody. Cytokeratins are a group of water-insoluble filamentous proteins. They are constituents of the cytoskeleton of epidermal cells and other epithelial cells. Up to now 20 different cytokeratins have been characterised by gel electrophoresis. They have been subdivided into a basic and an acid subfamily. Most frequently the nomenclature according to Moll et al. (1982) is used. Human cytokeratin 14. |
| Product Type | Antibodies Primary |
| Protocol | (lyophilisate) 1-10 µg/ml Western Blot, 0.5-1 µg/ml ELISA, IHC 2-10 µg/ml (not tested) |
| Purification | Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required. |
| Size | 1 ml |
| Source / Host | mouse |
| Storage | 2-8°C |
| Substrate / Buffer | Peptide Sepharose purified IgG in PBS, BSA, lyophilized without preservative. Reconstitute with 0.1 ml sterile water to get an antibody concentration of 1 mg/ml. Dilution buffers are depending on the type of application. |
| Technical Specifications | IHC(C, P) |
| UniProt Number | P02533 |
| Product Page Updated | 2023-12-29T14:25:47.027Z |
