Antibodies Secondary

Donkey Chicken IgY

NEW
Donkey Chicken IgY, conjugated with Agarose

Article No

303-DAIgY-AGA-1

Size

1.0 ml bed volume

Source / Host

other mammals

Specifications

Article No 303-DAIgY-AGA-1
Description Donkey Chicken IgY, conjugated with Agarose
Supplier Exalpha Biologicals
Format Gel
Notes A donkey was immunized with purified chicken IgY. Antibodies were isolated from immune sera using affinity chromatography and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites.
Product Type Antibodies Secondary
Protocol The specific activity of the anti-IgY-agarose was determined by applying 2.7 mg IgY in 24 ml PBS (0.1 mg/ml) to 1.0 ml bed volume of agarose. Two ml aliquots were collected. The total amount of IgY that bound to the column was calculated. For this lot, 1.3 mg IgY bound per ml agarose.
References 1. Hall, Sarah E., Song Luo, Anne E. Hall, and Daphne Preuss, 2005, Differential Rates of Local and Global Homogenization in Centromere Satellites From Arabidopsis Relatives. Genetics: 170(4): 1913–1927. 2. Song, M-G and M. Kiledjian, 2007. 3' Terminal oligo U-tract-mediated stimulation of decapping. RNA, 13:2356-2365. 3. Taylor, A.I., H.J. Gould, B.J. Sutton and R.A. Calvert. 2008. Avian IgY Binds to a Monocyte Receptor with IgG-like Kinetics Despite an IgE-like Structure. J. Biol. Chem. 283: 16384-16390. 4. S-C. Lo and M. Hannink. 2008. PGAM5 tethers a ternary complex containing Keap1 and Nrf2 to mitochondria. Experimental Cell Research 314: 1789-1803. 5. C. Butkinaree, W.D. Cheung, S. Park, K. Park, M. Barber and G.W. Hart, 2008. Characterization of Beta-N-Acetylglucosaminidase Cleavage by Caspase-3 during Apoptosis. J. Biol. Chem., 283:23557-23566. 6. Slawson,C. Lakshmanan, T., Knapp, S. and Hart, G.W., 2008, A Mitotic GlcNAcylation/Phosphorylation Signaling Complex Alters the Posttranslational State of the Cytoskeletal Protein Vimentin. Mol. Biol. Cell 19:4130-4140. 7. McMichael, Brooke K., Cheney, Richard E., and Beth S. Lee. 2010. Myosin X Regulates Sealing Zone Patterning in Osteoclasts through Linkage of Podosomes and Microtubules. J Biol. Chem. 285:9506-9515. 8. Hale, C.R., Sonali, M., Elmore, J., Pfister, N., Compton, M., Olson S., Resch, A.M., Glover III, C.V.C., Graveley B.R., Terns, R.M., and M.P. Terns. 2012. Essential Features and Rational Design of CRISPR RNAs that Function with the Cas RAMP Module Complex to Cleave RNAs. Molecular Cell 45:292-302. 9. Lehman, HL, van Laere SJ, van Golen, CM, Vermeulen PB, Dirix LY and KL van Golen. 2012. Regulation of inflammatory breast cancer cell invasion through Akt1/PKB? phosphorylation of RhoC GTPase. Mol Cancer Res. 10:1306-18. 10. Cheadle L. and T. Biederer. 2012. The novel synaptogenic protein Farp1 links postsynaptic cytoskeletal dynamics and transsynaptic organization. J Cell Biol. 199:985-1001. 11. Dai, L, Taylor, MS, O'Donnell, KA and JD Boeke. 2012. Poly (A) Binding Protein C1 is Essential for Efficient L1 Retrotransposition and Affects L1 RNP Formation. Mol Cell Biol. 32:4323-4336. 12. Joglekar, M, Elbazanti, WO, Weizman, MD, Lehman, HL and van Golen, KL. 2015. Caveolin-1 Mediates Inflammatory Breast Cancer Cell Invasion via the Akt1 Pathway and RhoC GTPase. J. Cell. Biochem 116:923-933. 13. Zhang, Z., Costa, FC, Tan, EP, Bushue, N, DiTacchio, N, Costello, CE, McComb, ME, Whelan, SA, Petersen, KR, and Slawson, C. 2016. O-Linked N-Acetylglucosamine (O-GlcNAc) Transferase and O-GlcNAcase Interact with Mi2? Protein at the A?-Globin Promoter. J. Biol Chem 291:15628-40. 14. Balachandran RS, Heighington CS, Starostina NG, Anderson JW, Owen DL, Vasudevan S, Kipreos ET. 2016. The ubiquitin ligase CRL2ZYG11 targets cyclin B1 for degradation in a conserved pathway that facilitates mitotic slippage. J Cell Biol. 215(2):151-166.
Size 1.0 ml bed volume
Source / Host other mammals
Storage 4°C in the dark. This product has a shelf life of at least 12 months.
Substrate / Buffer Gel; 1 ml settled gel
Technical Specifications The specific activity of the anti-IgY-agarose was determined by applying 2.7 mg IgY in 24 ml PBS (0.1 mg/ml) to 1.0 ml bed volume of agarose. Two ml aliquots were collected. The total amount of IgY that bound to the column was calculated. For this lot, 1.3 mg IgY bound per ml agarose.
Product Page Updated 2019-01-09T10:09:28.847Z

Shipping info

The delivery time for this item is approximately 5-8 business days. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.