Article No
303-MUB0604P
Mouse anti M13 phage coat protein g8p
Mouse anti M13 phage coat protein g8p
Antibodies Primary
Specifications
| Application | FC, IHC, IP, ICC |
| Article No | 303-MUB0604P |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | RL-ph2 |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | Mouse anti M13 phage coat protein g8p |
| Supplier | Exalpha Biologicals |
| Immunogen | RL-ph2 is a Mouse monoclonal IgG2a, _ antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with isolated M13 phage coat proteins. |
| Isotype | IgG2a |
| Notes | The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over Mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from Human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. |
| Product Type | Antibodies Primary |
| Protocol | RL-ph2 is particularly useful for immunoblotting of separated phage proteins, and is also suitable for detection of M13 phages in multistep immunoblotting procedures, immunocytochemistry, flow cytometry, affinity chromatography and ELISA. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:200 for flow cytometry, and 1:100 – 1:1000 for immunoblotting applications. |
| Purification | ProtG affinity purification |
| Research area | Microbiology |
| Shipping Information | Ship at ambient temperature. |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | bacteria |
| Storage | The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. |
| Substrate / Buffer | Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. |
| Technical Specifications | RL-ph2 is particularly useful for immunoblotting of sepaRated phage proteins, and is also suitable for detection of M13 phages in multistep immunoblotting procedures, immunocytochemistry, flow cytometry, affinity chromatography and ELISA. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:200 for flow cytometry, and 1:100 – 1:1000 for immunoblotting applications. |
| UniProt Number | P69541 |
| Product Page Updated | 2023-12-29T14:25:47.027Z |
