Article No
303-MUB1813P
Mouse anti Transferrin Receptor (CD71)
Mouse anti Transferrin Receptor (CD71)
Antibodies Primary
Specifications
| Application | IP, WB |
| Article No | 303-MUB1813P |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | 66IG10 |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | Mouse anti Transferrin Receptor (CD71) |
| Supplier | Exalpha Biologicals |
| Immunogen | 66IG10 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with Human T-cells. |
| Isotype | IgG1 |
| Notes | Transferrin receptor protein 1 (TfR1) also known as CD71 (Cluster of Differentiation 71) is a protein that in Humans is encoded by the TFRC gene. CD71 plays a critical role in cell prolifeRation by controlling the supply of iron, which is essential for many metabolic pathways, through the binding and endocytosis of transferrin, the major iron-carrying protein. The expression of CD71 is regulated at the post-transcriptional level through the control of mRNA stability and is closely linked to intracellular iron levels. Nitric oxide can affect CD71 expression independently of intracellular iron concentRation by activating proteins which then stabilize CD71 mRNA. Expression of CD71, or the transferrin receptor, is very low on resting leukocytes but is upregulated upon activation reflecting the iron dependence of activation. In other tissues, CD71 is expressed on most dividing cells and also on brain endothelium and alveolar macrophage. The ligand is the Serum iron transport protein transferrin. CD71 antibodies are used as an indiCator of prolifeRation in the typing of lymphomas and leukemias. |
| Product Type | Antibodies Primary |
| Protocol | 66IG10 is useful for western blot (on non reduced antigens) and immunoprecipitation, flow cytometry and immunohistochemsitry on frozen sections. Optimal antibody dilution should be determined by titration. |
| Research area | Immunology |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. |
| Substrate / Buffer | Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. |
| Technical Specifications | 66IG10 is useful for western blot (on non reduced antigens) and immunoprecipitation, flow cytometry and immunohistochemsitry on frozen sections. Optimal antibody dilution should be determined by titration. |
| UniProt Number | P02786 |
| Product Page Updated | 2023-12-29T14:25:47.027Z |
