Article No
303-SwAHu/IgG(Fc)/7S
Swine anti Human IgG (Fc specific)
Swine anti Human IgG (Fc specific)
Antibodies Secondary
Specifications
| Article No | 303-SwAHu/IgG(Fc)/7S |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | Polyclonal |
| Clone Type | polyclonal |
| Conjugation | Unconjugated |
| Description | Swine anti Human IgG (Fc specific) |
| Supplier | Exalpha Biologicals |
| Notes | The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal human plasma and serum, a characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No reaction is obtained with purified IgA, IgM and IgG/Fab fragments or any other serum protein. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. |
| Product Type | Antibodies Secondary |
| Protocol | Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting. |
| Size | 10 mg |
| Source / Host | pig/porcine |
| Species Reactivity | human |
| Storage | The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. |
| Substrate / Buffer | Purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
| Technical Specifications | Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting. |
| Product Page Updated | 2023-12-29T14:25:47.027Z |