Accessories

5x Annexin V Binding Buffer

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5x Annexin V Binding Buffer

Article No

4695-300

Size

50 mL

Shipping Information

4°C

Product insert

Specifications

Article No 4695-300
Country Availability SE, FI, DK, NO, IS, EE, LV, LT, FO, GL, RU
Description 5x Annexin V Binding Buffer
Supplier MBL International Corp.
Notes Apoptosis, a term that describes regulated cell death, is a fundamental feature of many processes including normal development, homeostasis, and disease. Early during the process of apoptosis, cells lose their phospholipid membrane asymmetry and expose phosphatidylserine (PS) at the cell surface. This process can be monitored by using Annexin V which is a Ca2+-dependent, phospholipid-binding protein with high affinity for PS that is useful for identifying apoptotic cells with exposed PS. Translocation of PS to the external cell surface is not unique to apoptosis, but occurs also during cell necrosis. The difference between these two forms of cell death is that during the initial stages of apoptosis the cell membrane remains intact, while when necrosis occurs the cell membrane loses its integrity and becomes leaky. Therefore, necrotic cells are easily stained with Propidium Iodide (PI) as well as Annexin V, where as Apoptotic cells stain only with Annexin V.
Product Type Accessories
Research Area Cell Death
Shipping Information 4°C
Size 50 mL
Stability 2 years
Storage 4°C
Substrate / Buffer 50mM HEPES/NaOH pH7.4750mM NaCl25mM KCl5mMMgCl29mM CaCl2
Technical Specifications Apoptosis, a term that describes regulated cell death, is a fundamental feature of many processes including normal development, homeostasis, and disease. Early during the process of apoptosis, cells lose their phospholipid membrane asymmetry and expose phosphatidylserine (PS) at the cell surface. This process can be monitored by using Annexin V which is a Ca2+-dependent, phospholipid-binding protein with high affinity for PS that is useful for identifying apoptotic cells with exposed PS. Translocation of PS to the external cell surface is not unique to apoptosis, but occurs also during cell necrosis. The difference between these two forms of cell death is that during the initial stages of apoptosis the cell membrane remains intact, while when necrosis occurs the cell membrane loses its integrity and becomes leaky. Therefore, necrotic cells are easily stained with Propidium Iodide (PI) as well as Annexin V, where as Apoptotic cells stain only with Annexin V.
Product Page Updated 2020-06-10T09:56:05.519Z

Shipping info

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