Article No
4800
Assay & Detection
APOPCYTO Caspase-3 Colorimetric Assay Kit
APOPCYTO Caspase-3 Colorimetric Assay Kit
Product insert
Specifications
| Article No | 4800 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL, RU |
| Components | Cell Lysis Buffer, 2X Reaction Buffer, DTT, Substrate DEVD-pNA, Inhibitor DEVD-FMK, pNA (p-nitroanilide) |
| Description | APOPCYTO Caspase-3 Colorimetric Assay Kit |
| Supplier | MBL International Corp. |
| Additional Information | Caspases are members of the cysteine aspartic acid-specific protease family, which may be activated by a variety of signals including death receptor ligation, DNA damage, serum starvation, stress, and many more. Active caspases recognize a wide number of different substrates, leading to the activation of several different apoptotic pathways. For example, CAD (caspase-activated deoxyribonuclease), a protein involved in chromatin fragmentation into nucleosome units, is indirectly activated by caspase-3, while ICAD (inhibitor of caspase-activated deoxyribonuclease) is inactivated by caspase-3. The cleavage of several structural proteins by caspases leads to the unique apoptosis cell morphology of chromatin condensation, nucleus fragmentation and cytoplasmic integrity. Detection of caspase activity is recognized as a standard method to measure apoptosis activity. The most common method to measure caspase activity is via the fluorometric or colorimetric detection of the cleavage of caspase-specific substrates. |
| Notes | Caspases are members of the cysteine aspartic acid-specific protease family, which may be activated by a variety of signals including death receptor ligation, DNA damage, serum starvation, stress, and many more. Active caspases recognize a wide number of different substrates, leading to the activation of several different apoptotic pathways. For example, CAD (caspase-activated deoxyribonuclease), a protein involved in chromatin fragmentation into nucleosome units, is indirectly activated by caspase-3, while ICAD (inhibitor of caspase-activated deoxyribonuclease) is inactivated by caspase-3. The cleavage of several structural proteins by caspases leads to the unique apoptosis cell morphology of chromatin condensation, nucleus fragmentation and cytoplasmic integrity. Detection of caspase activity is recognized as a standard method to measure apoptosis activity. The most common method to measure caspase activity is via the fluorometric or colorimetric detection of the cleavage of caspase-specific substrates. |
| Product Type | Assay & Detection |
| References | 1). Adachi M, et al., Clin Cancer Res, 10, 3853 (2004) 2). Brigotti M, et al., Infect Immun, 75, 2201 (2007) 3). Dinnen RD, et al., J Biol Chem, 282, 26675 (2007) 4). Goga Y, et al., J Dent Res, 85, 240 (2006) 5). Ishii T, et al., Cancer Res, 65, 203 (2005) 6). Madsen-Bouterse SA, et al., Endocrinology, 147, 3826 (2006) 7). Min Y, et al., Gut, 54, 591 (2005) 8). Nozaki N, et al., Circulation, 110, 2869 (2004) 9). Numata K, et al., J Immunol, 178, 3777 (2007) 10). Sinicrope FA, et al., Clin Cancer Res, 10, 8284 (2004) |
| Research Area | Cell Death |
| Shipping Information | dry ice |
| Size | 100 Assays |
| Stability | see label |
| Storage | -20°C. Please refer to datasheet for additional information |
| Technical Specifications | Caspases are members of the cysteine aspartic acid-specific protease family, which may be activated by a variety of signals including death receptor ligation, DNA damage, serum starvation, stress, and many more. Active caspases recognize a wide number of different substrates, leading to the activation of several different apoptotic pathways. For example, CAD (caspase-activated deoxyribonuclease), a protein involved in chromatin fragmentation into nucleosome units, is indirectly activated by caspase-3, while ICAD (inhibitor of caspase-activated deoxyribonuclease) is inactivated by caspase-3. The cleavage of several structural proteins by caspases leads to the unique apoptosis cell morphology of chromatin condensation, nucleus fragmentation and cytoplasmic integrity. Detection of caspase activity is recognized as a standard method to measure apoptosis activity. The most common method to measure caspase activity is via the fluorometric or colorimetric detection of the cleavage of caspase-specific substrates. |
| Product Page Updated | 2021-01-11T15:22:00.814Z |
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