Assay & Detection

APOPCYTO Caspase-9 Fluorometric Assay Kit

NEW
APOPCYTO Caspase-9 Fluorometric Assay Kit

Article No

4825

Size

100 Assays

Shipping Information

dry ice

Product insert

Specifications

Article No 4825
Country Availability SE, FI, DK, NO, IS, EE, LV, LT, FO, GL, RU
Components Cell Lysis Buffer, 2 X Reaction Buffer, 1M DTT, Substrate LEHD-AMC (2 mM), Inhibitor LEHD-FMK (1mM), 10 mM AMC (7-amino-4-methyl-coumarin)
Description APOPCYTO Caspase-9 Fluorometric Assay Kit
Supplier MBL International Corp.
Additional Information Caspases are members of the cysteine aspartic acid-specific protease family, which may be activated by a variety of signals including death receptor ligation, DNA damage, serum starvation, stress, and many more. Active caspases recognize a wide number of different substrates, leading to the activation of several different apoptotic pathways. For example, CAD (caspase-activated deoxyribonuclease), a protein involved in chromatin fragmentation into nucleosome units, is indirectly activated by caspase-3, while ICAD (inhibitor of caspase-activated deoxyribonuclease) is inactivated by caspase-3. The cleavage of several structural proteins by caspases leads to the unique apoptosis cell morphology of chromatin condensation, nucleus fragmentation and cytoplasmic integrity. Detection of caspase activity is recognized as a standard method to measure apoptosis activity. The most common method to measure caspase activity is via the fluorometric or colorimetric detection of the cleavage of caspase-specific substrates.
Notes Caspases are members of the cysteine aspartic acid-specific protease family, which may be activated by a variety of signals including death receptor ligation, DNA damage, serum starvation, stress, and many more. Active caspases recognize a wide number of different substrates, leading to the activation of several different apoptotic pathways. For example, CAD (caspase-activated deoxyribonuclease), a protein involved in chromatin fragmentation into nucleosome units, is indirectly activated by caspase-3, while ICAD (inhibitor of caspase-activated deoxyribonuclease) is inactivated by caspase-3. The cleavage of several structural proteins by caspases leads to the unique apoptosis cell morphology of chromatin condensation, nucleus fragmentation and cytoplasmic integrity. Detection of caspase activity is recognized as a standard method to measure apoptosis activity. The most common method to measure caspase activity is via the fluorometric or colorimetric detection of the cleavage of caspase-specific substrates.
Product Type Assay & Detection
References 1). Nanbo A, et al., J Virol, 79, 12280 (2005) 2). Okada M, et al., Blood, 103, 2299 (2004) 3). Shibata MA, et al., Carcinogenesis, 25, 1887 (2004) 4). Tsuruma K, et al., J Biol Chem, 281, 11397 (2006)
Research Area Cell Death
Shipping Information dry ice
Size 100 Assays
Stability see label
Storage -20°C. Please refer to datasheet for additional information
Technical Specifications Caspases are members of the cysteine aspartic acid-specific protease family, which may be activated by a variety of signals including death receptor ligation, DNA damage, serum starvation, stress, and many more. Active caspases recognize a wide number of different substrates, leading to the activation of several different apoptotic pathways. For example, CAD (caspase-activated deoxyribonuclease), a protein involved in chromatin fragmentation into nucleosome units, is indirectly activated by caspase-3, while ICAD (inhibitor of caspase-activated deoxyribonuclease) is inactivated by caspase-3. The cleavage of several structural proteins by caspases leads to the unique apoptosis cell morphology of chromatin condensation, nucleus fragmentation and cytoplasmic integrity. Detection of caspase activity is recognized as a standard method to measure apoptosis activity. The most common method to measure caspase activity is via the fluorometric or colorimetric detection of the cleavage of caspase-specific substrates.
Product Page Updated 2020-06-10T09:56:05.519Z

Shipping info

The delivery time for this item is approximately 8-15 business days. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.